PKC delta (PKCδ) promotes tumoral progression of human ductal pancreatic cancer

Laura V. Mauro, Valeria C. Grossoni, Alejandro J. Urtreger, Chengfeng Yang, Lucas L. Colombo, Ana Morandi, María G. Pallotta, Marcelo G. Kazanietz, Elisa D. Bal De Kier Joffé, Lydia L. Puricelli

Research output: Contribution to journalArticlepeer-review

52 Scopus citations


Objective: Our Objective was to study the role of protein kinase C delta (PKCδ) in the progression of human pancreatic carcinoma. Methods: Protein kinase C delta expression in human ductal carcinoma (n = 22) was studied by immunohistochemistry. We analyzed the effect of PKCδ overexpression on in vivo and in vitro properties of human ductal carcinoma cell line PANC1. Results: Human ductal carcinomas showed PKCδ overexpression compared with normal counterparts. In addition, in vitro PKCδ-PANC1 cells showed increased anchorage-independent growth and higher resistance to serum starvation and to treatment with cytotoxic drugs. Using pharmacological inhibitors, we determined that phosphatidylinositol-3-kinase and extracellular receptor kinase pathways were involved in the proliferation of PKCδ-PANC1. Interestingly, PKCδ-PANC1 cells showed a less in vitro invasive ability and an impairment in their ability to migrate and to secrete the proteolytic enzyme matrix metalloproteinase-2. In vivo experiments indicated that PKCδ-PANC1 cells were more tumorigenic, as they developed tumors with a significantly lower latency and a higher growth rate with respect to the tumors generated with control cells. Besides, only PKCδ-PANC1 cells developed lung metastasis. Conclusion: Our Results showed that the overexpression of PKCδ in PANC1 cells induced a more malignant phenotype in vivo, probably through the modulation of cell proliferation and survival, involving phosphatidylinositol-3- kinase and extracellular receptor kinase signaling pathways.

Original languageEnglish
Pages (from-to)e31-e41
Issue number1
StatePublished - Jan 2010


  • ERK
  • Human pancreatic adenocarcinoma
  • Invasion
  • PI3K/AKT
  • PKCC

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism
  • Hepatology
  • Endocrinology


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