Plasmodesmata Localizing Proteins Regulate Transport and Signaling during Systemic Acquired Immunity in Plants

Gah Hyun Lim; M. B. Shine; Laura De Lorenzo; Keshun Yu; Weier Cui; Duroy Navarre; Arthur G. Hunt; Jung Youn Lee; Aardra Kachroo; Pradeep Kachroo

doi:10.1016/j.chom.2016.03.006

Plasmodesmata Localizing Proteins Regulate Transport and Signaling during Systemic Acquired Immunity in Plants

Gah Hyun Lim, M. B. Shine, Laura De Lorenzo, Keshun Yu, Weier Cui, Duroy Navarre, Arthur G. Hunt, Jung Youn Lee, Aardra Kachroo, Pradeep Kachroo

Research output: Contribution to journal › Article › peer-review

131 Scopus citations

Abstract

Systemic acquired resistance (SAR) in plants is mediated by the signaling molecules azelaic acid (AzA), glycerol-3-phosphate (G3P), and salicylic acid (SA). Here, we show that AzA and G3P transport occurs via the symplastic route, which is regulated by channels known as plasmodesmata (PD). In contrast, SA moves via the extracytosolic apoplast compartment. We found that PD localizing proteins (PDLP) 1 and 5 were required for SAR even though PD permeability in pdlp1 and 5 mutants was comparable to or higher than wild-type plants, respectively. Furthermore, PDLP function was required in the recipient cell, suggesting regulatory function in SAR. Interestingly, overexpression of PDLP5 drastically reduced PD permeability, yet also impaired SAR. PDLP1 interacted with AZI1 (lipid transfer-like protein required for AzA- and G3P-induced SAR) and contributed to its intracellular partitioning. Together, these results reveal the transport routes of SAR chemical signals and highlight the regulatory role of PD-localizing proteins in SAR.

Original language	English
Pages (from-to)	541-549
Number of pages	9
Journal	Cell Host and Microbe
Volume	19
Issue number	4
DOIs	https://doi.org/10.1016/j.chom.2016.03.006
State	Published - Apr 13 2016

Bibliographical note

Publisher Copyright:
© 2016 Elsevier Inc.

Funding

This work was supported by grants from the National Science Foundation ( IOS#0749731 , #1457121 , #0954931 ) and a NSF grant under Cooperative Agreement No. 1355438 . We thank Andrea Pitzschke for AZI1-MYC transgenic plants, Arabidopsis database for PDLP knockout seeds, Xiangnan Guan for help with isolation of pdlp mutants, John Johnson for help with gas chromatography, Timothy Hoey for genotyping, Wendy Havens and Ludmila Lapchyk for technical help, and Amy Crume for managing the plant growth facility.

Funders	Funder number
National Science Foundation Arctic Social Science Program	IOS#0749731, 1457121, 0954931
Office of the Director	1355438

ASJC Scopus subject areas

Parasitology
Microbiology
Virology

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title = "Plasmodesmata Localizing Proteins Regulate Transport and Signaling during Systemic Acquired Immunity in Plants",

abstract = "Systemic acquired resistance (SAR) in plants is mediated by the signaling molecules azelaic acid (AzA), glycerol-3-phosphate (G3P), and salicylic acid (SA). Here, we show that AzA and G3P transport occurs via the symplastic route, which is regulated by channels known as plasmodesmata (PD). In contrast, SA moves via the extracytosolic apoplast compartment. We found that PD localizing proteins (PDLP) 1 and 5 were required for SAR even though PD permeability in pdlp1 and 5 mutants was comparable to or higher than wild-type plants, respectively. Furthermore, PDLP function was required in the recipient cell, suggesting regulatory function in SAR. Interestingly, overexpression of PDLP5 drastically reduced PD permeability, yet also impaired SAR. PDLP1 interacted with AZI1 (lipid transfer-like protein required for AzA- and G3P-induced SAR) and contributed to its intracellular partitioning. Together, these results reveal the transport routes of SAR chemical signals and highlight the regulatory role of PD-localizing proteins in SAR.",

author = "Lim, {Gah Hyun} and Shine, {M. B.} and {De Lorenzo}, Laura and Keshun Yu and Weier Cui and Duroy Navarre and Hunt, {Arthur G.} and Lee, {Jung Youn} and Aardra Kachroo and Pradeep Kachroo",

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AU - Shine, M. B.

AU - De Lorenzo, Laura

AU - Yu, Keshun

AU - Cui, Weier

AU - Navarre, Duroy

AU - Hunt, Arthur G.

AU - Lee, Jung Youn

AU - Kachroo, Aardra

AU - Kachroo, Pradeep

PY - 2016/4/13

Y1 - 2016/4/13

N2 - Systemic acquired resistance (SAR) in plants is mediated by the signaling molecules azelaic acid (AzA), glycerol-3-phosphate (G3P), and salicylic acid (SA). Here, we show that AzA and G3P transport occurs via the symplastic route, which is regulated by channels known as plasmodesmata (PD). In contrast, SA moves via the extracytosolic apoplast compartment. We found that PD localizing proteins (PDLP) 1 and 5 were required for SAR even though PD permeability in pdlp1 and 5 mutants was comparable to or higher than wild-type plants, respectively. Furthermore, PDLP function was required in the recipient cell, suggesting regulatory function in SAR. Interestingly, overexpression of PDLP5 drastically reduced PD permeability, yet also impaired SAR. PDLP1 interacted with AZI1 (lipid transfer-like protein required for AzA- and G3P-induced SAR) and contributed to its intracellular partitioning. Together, these results reveal the transport routes of SAR chemical signals and highlight the regulatory role of PD-localizing proteins in SAR.

AB - Systemic acquired resistance (SAR) in plants is mediated by the signaling molecules azelaic acid (AzA), glycerol-3-phosphate (G3P), and salicylic acid (SA). Here, we show that AzA and G3P transport occurs via the symplastic route, which is regulated by channels known as plasmodesmata (PD). In contrast, SA moves via the extracytosolic apoplast compartment. We found that PD localizing proteins (PDLP) 1 and 5 were required for SAR even though PD permeability in pdlp1 and 5 mutants was comparable to or higher than wild-type plants, respectively. Furthermore, PDLP function was required in the recipient cell, suggesting regulatory function in SAR. Interestingly, overexpression of PDLP5 drastically reduced PD permeability, yet also impaired SAR. PDLP1 interacted with AZI1 (lipid transfer-like protein required for AzA- and G3P-induced SAR) and contributed to its intracellular partitioning. Together, these results reveal the transport routes of SAR chemical signals and highlight the regulatory role of PD-localizing proteins in SAR.

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Plasmodesmata Localizing Proteins Regulate Transport and Signaling during Systemic Acquired Immunity in Plants

Abstract

Bibliographical note

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