Plastidial fatty acid levels regulate resistance gene-dependent defense signaling in Arabidopsis

A. C. Chandra-Shekara, Srivathsa C. Venugopal, Subhankar Roy Barman, Aardra Kachroo, Pradeep Kachroo

Research output: Contribution to journalArticlepeer-review

81 Scopus citations

Abstract

In Arabidopsis, resistance to Turnip Crinkle Virus (TCV) depends on the resistance (R) gene, HRT, and the recessive locus rrt. Resistance also depends on salicylic acid (SA), EDS1, and PAD4. Exogenous application of SA confers resistance in PRT-containing plants by increasing HRT transcript levels in a PAD4-dependent manner. Here we report that reduction of oleic acid (18:1) can also induce HRT gene expression and confer resistance to TCV. However, the 18:1-regulated pathway is independent of SA, rrt, EDS1, and PAD4. Reducing the levels of 18:1, via a mutation in the SSI2-encoded stearoyl-acyl carrier protein-desaturase, or by exogenous application of glycerol, increased transcript levels of HRT as well as several other R genes. Second-site mutations in the ACT1-encoded glycerol-3-phosphate acyltransferase or GLY1-encoded glycerol-3-phosphate dehydrogenase restored 18:1 levels in HRT ssi2 plants and reestablished a dependence on rrt. Resistance to TCV and HRT gene expression in HRT act1 plants was inducible by SA but not by glycerol, whereas that in HRT pad4 plants was inducible by glycerol but not by SA. The low 18:1-mediated induction of R gene expression was also dependent on ACT1 but independent of EDS1, PAD4, and RAR1. Intriguingly, TCV inoculation did not activate this 18:1-regulated pathway in HRT plants, but instead resulted in the induction of several genes that encode 18:1-synthesizing isozymes. These results suggest that the 18:1-regulated pathway may be specifically targeted during pathogen infection and that altering 18:1 levels may serve as a unique strategy for promoting disease resistance.

Original languageEnglish
Pages (from-to)7277-7282
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume104
Issue number17
DOIs
StatePublished - Apr 24 2007

Keywords

  • Glycerol
  • Salicylic acid
  • Stearoyl-acyl carrier protein-desaturase
  • Turnip Crinkle Virus
  • ssi2

ASJC Scopus subject areas

  • General

Fingerprint

Dive into the research topics of 'Plastidial fatty acid levels regulate resistance gene-dependent defense signaling in Arabidopsis'. Together they form a unique fingerprint.

Cite this