Platelet factor 4 induces human natural killer cells to synthesize and release interleukin-8

Francesc Martí, Esther Bertran, Montserrat Llucià, Esther Villén, Matilde Peiró, Joan Garcia, Fèlix Rueda

Research output: Contribution to journalArticlepeer-review

32 Scopus citations


We provide evidence that platelet factor 4 (PF4), but not the related chemokine neutrophil-activating polypeptide-2, induced highly purified human natural killer (NK) cells to produce interleukin (IL)-8 in a time- and dosage-dependent manner. This ability was retained even while PF4 was bound to heparin. PF4 increased the steady state level of IL-8 mRNA, likely implying a transcriptional effect of PF4. Stimulation of NK cells through the Fc receptor for immunoglobulin G-IIIA was found to synergistically increase the effect of PF4 on IL-8 production but did not affect IL-2-related activities such as cytotoxic activity and proliferation. Pertussis toxin did not block the PF4-derived IL-8 production in NK cells, but this response was sensitive to wortmannin, implicating a role of phosphatidylinositol 3-kinase in the intracellular signaling pathway triggered by PF4. Our results characterize a new capacity for PF4 and provide further evidence for the pivotal role of NK cells in the environment of inflammation.

Original languageEnglish
Pages (from-to)590-597
Number of pages8
JournalJournal of Leukocyte Biology
Issue number3
StatePublished - Sep 1 2002


  • Chemokines
  • FcγRIIIA
  • Inflammation

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Cell Biology


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