Plk1 phosphorylation of Topors is involved in its degradation

Xiaoming Yang; Hongchang Li; Anping Deng; Xiaoqi Liu

doi:10.1007/s11033-009-9871-1

Plk1 phosphorylation of Topors is involved in its degradation

Xiaoming Yang
, Hongchang Li
, Anping Deng
, Xiaoqi Liu

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Topors is a DNA topoisomerase I- and p53-binding protein, and mainly functions as a p53 regulator. Accumulating evidence also supports the notion that Topors plays the role as a negative regulator of cell growth, and possibly as a tumor suppressor. Here, we demonstrated that Topors is also involved in normal mitotic progression, since Topors depletion delays mitotic entry and affects mitotic progression. Furthermore, Topors is degradated in response to the activation of the spindle checkpoint. Significantly, Polo-like kinase 1 (Plk1)-associated phosphorylation of Topors at S718 is essential for nocodazole-induced degradation of Topors.

Original language	English
Pages (from-to)	3023-3028
Number of pages	6
Journal	Molecular Biology Reports
Volume	37
Issue number	6
DOIs	https://doi.org/10.1007/s11033-009-9871-1
State	Published - Jul 2010

Bibliographical note

Funding Information:
Acknowledgments We thank Drs. Ourania Andrisani and Wen-Horng Wang for helpful discussions. X.L. is a recipient of the Howard Temin Award K01 CA114401 from the National Cancer Institute. X.Y. is supported by the China Scholarship Council.

Funding

Acknowledgments We thank Drs. Ourania Andrisani and Wen-Horng Wang for helpful discussions. X.L. is a recipient of the Howard Temin Award K01 CA114401 from the National Cancer Institute. X.Y. is supported by the China Scholarship Council.

Funders	Funder number
National Institutes of Health (NIH)
National Childhood Cancer Registry – National Cancer Institute	R01CA157429
China Scholarship Council

Keywords

Phosphorylation
Polo-like kinase 1
Protein degradation
Spindle checkpoint
Topors

ASJC Scopus subject areas

Molecular Biology
Genetics

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10.1007/s11033-009-9871-1

Cite this

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title = "Plk1 phosphorylation of Topors is involved in its degradation",

abstract = "Topors is a DNA topoisomerase I- and p53-binding protein, and mainly functions as a p53 regulator. Accumulating evidence also supports the notion that Topors plays the role as a negative regulator of cell growth, and possibly as a tumor suppressor. Here, we demonstrated that Topors is also involved in normal mitotic progression, since Topors depletion delays mitotic entry and affects mitotic progression. Furthermore, Topors is degradated in response to the activation of the spindle checkpoint. Significantly, Polo-like kinase 1 (Plk1)-associated phosphorylation of Topors at S718 is essential for nocodazole-induced degradation of Topors.",

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note = "Funding Information: Acknowledgments We thank Drs. Ourania Andrisani and Wen-Horng Wang for helpful discussions. X.L. is a recipient of the Howard Temin Award K01 CA114401 from the National Cancer Institute. X.Y. is supported by the China Scholarship Council.",

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AU - Yang, Xiaoming

AU - Li, Hongchang

AU - Deng, Anping

AU - Liu, Xiaoqi

N1 - Funding Information: Acknowledgments We thank Drs. Ourania Andrisani and Wen-Horng Wang for helpful discussions. X.L. is a recipient of the Howard Temin Award K01 CA114401 from the National Cancer Institute. X.Y. is supported by the China Scholarship Council.

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N2 - Topors is a DNA topoisomerase I- and p53-binding protein, and mainly functions as a p53 regulator. Accumulating evidence also supports the notion that Topors plays the role as a negative regulator of cell growth, and possibly as a tumor suppressor. Here, we demonstrated that Topors is also involved in normal mitotic progression, since Topors depletion delays mitotic entry and affects mitotic progression. Furthermore, Topors is degradated in response to the activation of the spindle checkpoint. Significantly, Polo-like kinase 1 (Plk1)-associated phosphorylation of Topors at S718 is essential for nocodazole-induced degradation of Topors.

AB - Topors is a DNA topoisomerase I- and p53-binding protein, and mainly functions as a p53 regulator. Accumulating evidence also supports the notion that Topors plays the role as a negative regulator of cell growth, and possibly as a tumor suppressor. Here, we demonstrated that Topors is also involved in normal mitotic progression, since Topors depletion delays mitotic entry and affects mitotic progression. Furthermore, Topors is degradated in response to the activation of the spindle checkpoint. Significantly, Polo-like kinase 1 (Plk1)-associated phosphorylation of Topors at S718 is essential for nocodazole-induced degradation of Topors.

KW - Phosphorylation

KW - Polo-like kinase 1

KW - Protein degradation

KW - Spindle checkpoint

KW - Topors

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Plk1 phosphorylation of Topors is involved in its degradation

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

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