TY - JOUR
T1 - Poly(ADP-ribose) polymerase-1 is required for efficient HIV-1 integration
AU - Ha, Hyo Chol
AU - Juluri, Krishna
AU - Zhou, Yan
AU - Leung, Steve
AU - Hermankova, Monika
AU - Snyder, Solomon H.
PY - 2001/3/13
Y1 - 2001/3/13
N2 - Poly(ADP-ribose) polymerase-1 (PARP-1; EC 2.4.2.30) is an abundant nuclear enzyme, activated by DNA strand breaks to attach up to 200 ADP-ribose groups to nuclear proteins. As retroviral infection requires integrase-catalyzed DNA strand breaks, we examined infection of pseudotyped HIV type I in fibroblasts from mice with a targeted deletion of PARP-1. Viral infection is almost totally abolished in PARP-1 knockout fibroblasts. This protection from infection reflects prevention of viral integration into the host genome. These findings suggest a potential for PARP inhibitors in therapy of HIV type I infection.
AB - Poly(ADP-ribose) polymerase-1 (PARP-1; EC 2.4.2.30) is an abundant nuclear enzyme, activated by DNA strand breaks to attach up to 200 ADP-ribose groups to nuclear proteins. As retroviral infection requires integrase-catalyzed DNA strand breaks, we examined infection of pseudotyped HIV type I in fibroblasts from mice with a targeted deletion of PARP-1. Viral infection is almost totally abolished in PARP-1 knockout fibroblasts. This protection from infection reflects prevention of viral integration into the host genome. These findings suggest a potential for PARP inhibitors in therapy of HIV type I infection.
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U2 - 10.1073/pnas.051633498
DO - 10.1073/pnas.051633498
M3 - Article
C2 - 11248084
AN - SCOPUS:0035853143
SN - 0027-8424
VL - 98
SP - 3364
EP - 3368
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 6
ER -