Poly(ADP-ribose) polymerase-1 is required for efficient HIV-1 integration

Hyo Chol Ha; Krishna Juluri; Yan Zhou; Steve Leung; Monika Hermankova; Solomon H. Snyder

doi:10.1073/pnas.051633498

Poly(ADP-ribose) polymerase-1 is required for efficient HIV-1 integration

Hyo Chol Ha, Krishna Juluri, Yan Zhou, Steve Leung, Monika Hermankova, Solomon H. Snyder

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89 Scopus citations

Abstract

Poly(ADP-ribose) polymerase-1 (PARP-1; EC 2.4.2.30) is an abundant nuclear enzyme, activated by DNA strand breaks to attach up to 200 ADP-ribose groups to nuclear proteins. As retroviral infection requires integrase-catalyzed DNA strand breaks, we examined infection of pseudotyped HIV type I in fibroblasts from mice with a targeted deletion of PARP-1. Viral infection is almost totally abolished in PARP-1 knockout fibroblasts. This protection from infection reflects prevention of viral integration into the host genome. These findings suggest a potential for PARP inhibitors in therapy of HIV type I infection.

Original language	English
Pages (from-to)	3364-3368
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	98
Issue number	6
DOIs	https://doi.org/10.1073/pnas.051633498
State	Published - Mar 13 2001

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10.1073/pnas.051633498

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title = "Poly(ADP-ribose) polymerase-1 is required for efficient HIV-1 integration",

abstract = "Poly(ADP-ribose) polymerase-1 (PARP-1; EC 2.4.2.30) is an abundant nuclear enzyme, activated by DNA strand breaks to attach up to 200 ADP-ribose groups to nuclear proteins. As retroviral infection requires integrase-catalyzed DNA strand breaks, we examined infection of pseudotyped HIV type I in fibroblasts from mice with a targeted deletion of PARP-1. Viral infection is almost totally abolished in PARP-1 knockout fibroblasts. This protection from infection reflects prevention of viral integration into the host genome. These findings suggest a potential for PARP inhibitors in therapy of HIV type I infection.",

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AU - Ha, Hyo Chol

AU - Juluri, Krishna

AU - Zhou, Yan

AU - Leung, Steve

AU - Hermankova, Monika

AU - Snyder, Solomon H.

PY - 2001/3/13

Y1 - 2001/3/13

N2 - Poly(ADP-ribose) polymerase-1 (PARP-1; EC 2.4.2.30) is an abundant nuclear enzyme, activated by DNA strand breaks to attach up to 200 ADP-ribose groups to nuclear proteins. As retroviral infection requires integrase-catalyzed DNA strand breaks, we examined infection of pseudotyped HIV type I in fibroblasts from mice with a targeted deletion of PARP-1. Viral infection is almost totally abolished in PARP-1 knockout fibroblasts. This protection from infection reflects prevention of viral integration into the host genome. These findings suggest a potential for PARP inhibitors in therapy of HIV type I infection.

AB - Poly(ADP-ribose) polymerase-1 (PARP-1; EC 2.4.2.30) is an abundant nuclear enzyme, activated by DNA strand breaks to attach up to 200 ADP-ribose groups to nuclear proteins. As retroviral infection requires integrase-catalyzed DNA strand breaks, we examined infection of pseudotyped HIV type I in fibroblasts from mice with a targeted deletion of PARP-1. Viral infection is almost totally abolished in PARP-1 knockout fibroblasts. This protection from infection reflects prevention of viral integration into the host genome. These findings suggest a potential for PARP inhibitors in therapy of HIV type I infection.

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Poly(ADP-ribose) polymerase-1 is required for efficient HIV-1 integration

Abstract

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