Polymorphism and conservation of the genes encoding Qa1 molecules

Evan Hermel, Andrew J. Hart, Irfan Gunduz, Hector Acton, Christina Kim, Mark Wurth, Samina Uddin, Caryn Smith, Kirsten Fischer Lindahl, Carla J. Aldrich

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

To evaluate the polymorphism and conservation of the major histocompatibility complex class Ib molecule Qa1 in wild mouse populations, we determined the nucleotide sequence of exons 1-3 of Qa1 of eight mouse haplotypes derived from wild mice, including Mus musculus domesticus, M. m. castaneus, M. m. bactrianus, and M. spretus, as well as two t haplotypes. Our data identify eight new alleles of Qa1. Taken together with previously published data on Qa1 among the common laboratory inbred strains, and in agreement with cytotoxic T-lymphocyte, serological, and biochemical data, these results further confirm the existence of two families of Qa1 molecules, Qa1a-like and Qa1b-like, and illuminate the extreme conservation of the peptide-binding region of these molecules, even across species.

Original languageEnglish
Pages (from-to)639-649
Number of pages11
JournalImmunogenetics
Volume56
Issue number9
DOIs
StatePublished - Dec 2004

Bibliographical note

Funding Information:
Acknowledgements We thank Carolyn (Lucy) Shuck and Amy Samm for expert technical assistance and Diana Fuqua, Margaretan Stolfo and Anne Eubanks for excellence in animal breeding and care. Cell irradiation was kindly provided by the Evansville Cancer Center. This work was supported in part by grant JFRA-645 from the American Cancer Society, and Indiana University Diabetes and Research Center NIH grant P60 DK20542 (CJA) and NIH grant AI37818 (KFL).

Keywords

  • Cytotoxic T lymphocyte
  • H2-Qa1
  • H2-T23
  • MHC class Ib
  • Mus spretus
  • Wild mice

ASJC Scopus subject areas

  • Immunology
  • Genetics

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