TY - JOUR
T1 - Poor mobilization in T-cell-deficient nude mice is explained by defective activation of granulocytes and monocytes
AU - Wysoczynski, Marcin
AU - Adamiak, Mateusz
AU - Suszynska, Malwina
AU - Abdel-Latif, Ahmed
AU - Ratajczak, Janina
AU - Ratajczak, Mariusz Z.
N1 - Publisher Copyright:
© 2017 Cognizant, LLC.
PY - 2017
Y1 - 2017
N2 - It has been reported that both SCID mice and SCID patients poorly mobilize hematopoietic stem/progenitor cells (HSPCs) in response to granulocyte colony-stimulating factor (G-CSF). This defect has been proposed to result from a lack of naturally occurring IgM immunoglobulins to trigger activation of the complement cascade (ComC) and release of C5 cleavage fragments crucial in the mobilization process. However, SCID individuals also have T-cell deficiency, and T cells have been shown to modulate trafficking of HSPCs. To learn more about the role of T lymphocytes, we performed mobilization studies in T-lymphocyte-deficient nude mice and found that these mice respond poorly to G-CSF and zymosan but are normal mobilizers in response to AMD3100. Since nude mice have normal levels of IgM immunoglobulins in peripheral blood and may activate the ComC, we focused on the potential involvement of Gr1+ granulocytes and monocytes, which show defective maturation in these animals. Using a nude mouse mobilization model, we found further support for the proposition that proper function of Gr1+ cells is crucial for optimal mobilization of HSPCs.
AB - It has been reported that both SCID mice and SCID patients poorly mobilize hematopoietic stem/progenitor cells (HSPCs) in response to granulocyte colony-stimulating factor (G-CSF). This defect has been proposed to result from a lack of naturally occurring IgM immunoglobulins to trigger activation of the complement cascade (ComC) and release of C5 cleavage fragments crucial in the mobilization process. However, SCID individuals also have T-cell deficiency, and T cells have been shown to modulate trafficking of HSPCs. To learn more about the role of T lymphocytes, we performed mobilization studies in T-lymphocyte-deficient nude mice and found that these mice respond poorly to G-CSF and zymosan but are normal mobilizers in response to AMD3100. Since nude mice have normal levels of IgM immunoglobulins in peripheral blood and may activate the ComC, we focused on the potential involvement of Gr1+ granulocytes and monocytes, which show defective maturation in these animals. Using a nude mouse mobilization model, we found further support for the proposition that proper function of Gr1+ cells is crucial for optimal mobilization of HSPCs.
KW - Complement
KW - Granulocyte degranulation
KW - Stem cell mobilization
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U2 - 10.3727/096368916X692221
DO - 10.3727/096368916X692221
M3 - Article
C2 - 27436627
AN - SCOPUS:85010375266
SN - 0963-6897
VL - 26
SP - 83
EP - 93
JO - Cell Transplantation
JF - Cell Transplantation
IS - 1
ER -