Population genetics of the predatory lady beetle Hippodamia convergens

Arun Sethuraman; Fredric J. Janzen; John Obrycki

doi:10.1016/j.biocontrol.2015.01.002

Population genetics of the predatory lady beetle Hippodamia convergens

Arun Sethuraman, Fredric J. Janzen, John Obrycki

Entomology

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

Quantifying non-target effects of augmentative releases on populations of conspecifics is key to understanding the long-term impacts of augmentation biological control. Potential deleterious (and advantageous) allelic variation carried over to augmented populations from 'source' populations could shape adaptive evolutionary trajectories. Variation at seven microsatellite loci was determined in 117 adults from 11 populations (2 populations from California, 1 from Arizona, 1 from South America, and 7 from regions east of the Rocky Mountains in the United States [hereafter, Eastern]) of the widely distributed predatory lady beetle, Hippodamia convergens Guerin (Coleoptera: Coccinellidae). Our study was designed to examine possible introgression of genes from adult H. convergens that are mass-collected in California annually and released in eastern North America for augmentative biological control. The average observed heterozygosity was 0.44 and all loci were polymorphic (mean. =. 20.57 alleles/locus). The number of genetically distinct subpopulations of H. convergens was estimated to be at least two. Our analyses indicate that Californian multilocus genotypes are admixed within Eastern populations of H. convergens. We also determined the sizes of California populations to be larger than all sampled Eastern populations, suggesting recent declines in the latter. Additional study of the population demography of H. convergens and its local ecological adaptations is required to determine if these augmentative releases are causing large-scale non-target effects.

Original language	English
Pages (from-to)	1-10
Number of pages	10
Journal	Biological Control
Volume	84
DOIs	https://doi.org/10.1016/j.biocontrol.2015.01.002
State	Published - May 1 2015

Bibliographical note

Funding Information:
This research was supported by a USDA-NIFA sabbatical grant ( 2011-67014-30194 ) to JJO. We thank Andy Michel and Mary Gardiner, Department of Entomology, Ohio Agricultural Research and Development Center, Wooster, Ohio for sharing their microsatellite markers.We thank Morgan Becker, Audra Loy, Chelsea Sawyers, Leighfonda Allen, Daniela Flores, other members of the Janzen Lab at Iowa State University, and Lalitha Sethuraman for assistance with genotyping. We are very grateful to all the collectors of H. convergens listed in Table 1 . We thank Dr. Ric Bessin, Department of Entomology, University of Kentucky, for the image of H. convergens . All analyses were performed on the Temple HPC (Owlsnest) which was supported in part by the NSF major research instrumentation grant number CNS-09-58854.

Publisher Copyright:
© 2015 Elsevier Inc.

Keywords

Augmentative release
Bayesian MCMC
Demography
Microsatellite
Population structure
Source-sink model

ASJC Scopus subject areas

Agronomy and Crop Science
Insect Science

Access to Document

10.1016/j.biocontrol.2015.01.002

Cite this

@article{a6759952b08a4bd09784a1c4c7b8d5a0,

title = "Population genetics of the predatory lady beetle Hippodamia convergens",

abstract = "Quantifying non-target effects of augmentative releases on populations of conspecifics is key to understanding the long-term impacts of augmentation biological control. Potential deleterious (and advantageous) allelic variation carried over to augmented populations from 'source' populations could shape adaptive evolutionary trajectories. Variation at seven microsatellite loci was determined in 117 adults from 11 populations (2 populations from California, 1 from Arizona, 1 from South America, and 7 from regions east of the Rocky Mountains in the United States [hereafter, Eastern]) of the widely distributed predatory lady beetle, Hippodamia convergens Guerin (Coleoptera: Coccinellidae). Our study was designed to examine possible introgression of genes from adult H. convergens that are mass-collected in California annually and released in eastern North America for augmentative biological control. The average observed heterozygosity was 0.44 and all loci were polymorphic (mean. =. 20.57 alleles/locus). The number of genetically distinct subpopulations of H. convergens was estimated to be at least two. Our analyses indicate that Californian multilocus genotypes are admixed within Eastern populations of H. convergens. We also determined the sizes of California populations to be larger than all sampled Eastern populations, suggesting recent declines in the latter. Additional study of the population demography of H. convergens and its local ecological adaptations is required to determine if these augmentative releases are causing large-scale non-target effects.",

keywords = "Augmentative release, Bayesian MCMC, Demography, Microsatellite, Population structure, Source-sink model",

author = "Arun Sethuraman and Janzen, {Fredric J.} and John Obrycki",

note = "Funding Information: This research was supported by a USDA-NIFA sabbatical grant ( 2011-67014-30194 ) to JJO. We thank Andy Michel and Mary Gardiner, Department of Entomology, Ohio Agricultural Research and Development Center, Wooster, Ohio for sharing their microsatellite markers.We thank Morgan Becker, Audra Loy, Chelsea Sawyers, Leighfonda Allen, Daniela Flores, other members of the Janzen Lab at Iowa State University, and Lalitha Sethuraman for assistance with genotyping. We are very grateful to all the collectors of H. convergens listed in Table 1 . We thank Dr. Ric Bessin, Department of Entomology, University of Kentucky, for the image of H. convergens . All analyses were performed on the Temple HPC (Owlsnest) which was supported in part by the NSF major research instrumentation grant number CNS-09-58854. Publisher Copyright: {\textcopyright} 2015 Elsevier Inc.",

year = "2015",

month = may,

day = "1",

doi = "10.1016/j.biocontrol.2015.01.002",

language = "English",

volume = "84",

pages = "1--10",

}

TY - JOUR

T1 - Population genetics of the predatory lady beetle Hippodamia convergens

AU - Sethuraman, Arun

AU - Janzen, Fredric J.

AU - Obrycki, John

N1 - Funding Information: This research was supported by a USDA-NIFA sabbatical grant ( 2011-67014-30194 ) to JJO. We thank Andy Michel and Mary Gardiner, Department of Entomology, Ohio Agricultural Research and Development Center, Wooster, Ohio for sharing their microsatellite markers.We thank Morgan Becker, Audra Loy, Chelsea Sawyers, Leighfonda Allen, Daniela Flores, other members of the Janzen Lab at Iowa State University, and Lalitha Sethuraman for assistance with genotyping. We are very grateful to all the collectors of H. convergens listed in Table 1 . We thank Dr. Ric Bessin, Department of Entomology, University of Kentucky, for the image of H. convergens . All analyses were performed on the Temple HPC (Owlsnest) which was supported in part by the NSF major research instrumentation grant number CNS-09-58854. Publisher Copyright: © 2015 Elsevier Inc.

PY - 2015/5/1

Y1 - 2015/5/1

N2 - Quantifying non-target effects of augmentative releases on populations of conspecifics is key to understanding the long-term impacts of augmentation biological control. Potential deleterious (and advantageous) allelic variation carried over to augmented populations from 'source' populations could shape adaptive evolutionary trajectories. Variation at seven microsatellite loci was determined in 117 adults from 11 populations (2 populations from California, 1 from Arizona, 1 from South America, and 7 from regions east of the Rocky Mountains in the United States [hereafter, Eastern]) of the widely distributed predatory lady beetle, Hippodamia convergens Guerin (Coleoptera: Coccinellidae). Our study was designed to examine possible introgression of genes from adult H. convergens that are mass-collected in California annually and released in eastern North America for augmentative biological control. The average observed heterozygosity was 0.44 and all loci were polymorphic (mean. =. 20.57 alleles/locus). The number of genetically distinct subpopulations of H. convergens was estimated to be at least two. Our analyses indicate that Californian multilocus genotypes are admixed within Eastern populations of H. convergens. We also determined the sizes of California populations to be larger than all sampled Eastern populations, suggesting recent declines in the latter. Additional study of the population demography of H. convergens and its local ecological adaptations is required to determine if these augmentative releases are causing large-scale non-target effects.

AB - Quantifying non-target effects of augmentative releases on populations of conspecifics is key to understanding the long-term impacts of augmentation biological control. Potential deleterious (and advantageous) allelic variation carried over to augmented populations from 'source' populations could shape adaptive evolutionary trajectories. Variation at seven microsatellite loci was determined in 117 adults from 11 populations (2 populations from California, 1 from Arizona, 1 from South America, and 7 from regions east of the Rocky Mountains in the United States [hereafter, Eastern]) of the widely distributed predatory lady beetle, Hippodamia convergens Guerin (Coleoptera: Coccinellidae). Our study was designed to examine possible introgression of genes from adult H. convergens that are mass-collected in California annually and released in eastern North America for augmentative biological control. The average observed heterozygosity was 0.44 and all loci were polymorphic (mean. =. 20.57 alleles/locus). The number of genetically distinct subpopulations of H. convergens was estimated to be at least two. Our analyses indicate that Californian multilocus genotypes are admixed within Eastern populations of H. convergens. We also determined the sizes of California populations to be larger than all sampled Eastern populations, suggesting recent declines in the latter. Additional study of the population demography of H. convergens and its local ecological adaptations is required to determine if these augmentative releases are causing large-scale non-target effects.

KW - Augmentative release

KW - Bayesian MCMC

KW - Demography

KW - Microsatellite

KW - Population structure

KW - Source-sink model

UR - http://www.scopus.com/inward/record.url?scp=84922463924&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84922463924&partnerID=8YFLogxK

U2 - 10.1016/j.biocontrol.2015.01.002

DO - 10.1016/j.biocontrol.2015.01.002

M3 - Article

AN - SCOPUS:84922463924

SN - 1049-9644

VL - 84

SP - 1

EP - 10

JO - Biological Control

JF - Biological Control

ER -

Population genetics of the predatory lady beetle Hippodamia convergens

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this