Porous EH and EH-PEG scaffolds as gene delivery vehicles to skeletal muscle

Erin E. Falco; Martha O. Wang; Joshua A. Thompson; Joshua M. Chetta; Diana M. Yoon; Erik Z. Li; Mangesh M. Kulkami; Sameer Shah; Abhay Pandit; J. Scott Roth; John P. Fisher

doi:10.1007/s11095-010-0358-5

Porous EH and EH-PEG scaffolds as gene delivery vehicles to skeletal muscle

Erin E. Falco, Martha O. Wang, Joshua A. Thompson, Joshua M. Chetta, Diana M. Yoon, Erik Z. Li, Mangesh M. Kulkami, Sameer Shah, Abhay Pandit, J. Scott Roth, John P. Fisher

Surgery

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

Purpose: Synthetic biomaterials are widely used in an attempt to control the cellular behavior of regenerative tissues. This can be done by altering the chemical and physical properties of the polymeric scaffold to guide tissue repair. This paper addresses the use of a polymeric scaffold (EH network) made from the cyclic acetal monomer, 5-ethyl-5-(hydroxymethyl)-β,β- dimethyl-1,3-dioxane-2-ethanol diacrylate (EHD), as a release device for a therapeutic plasmid encoding for an insulin-like growth factor-1 green fluorescent protein fusion protein (IGF-1 GFP). Methods: Scaffolds were designed to have different porous architectures, and the impact of these architectures on plasmid release was determined. We hypothesized that IGF-1 could be delivered more effectively using a porous scaffold to allow for the release of IGF-1. Results: We showed that by altering the number of pores exposed to the surface of the network, faster plasmid loading and release were achieved. In addition, the IGF-1 GFP plasmids were found to be effective in producing IGF-1 and GFP within human skeletal muscle myoblast cell cultures. Conclusions: This work aims to show the utility of EH biomaterials for plasmid delivery for potentially localized skeletal muscle regeneration.

Original language	English
Pages (from-to)	1306-1316
Number of pages	11
Journal	Pharmaceutical Research
Volume	28
Issue number	6
DOIs	https://doi.org/10.1007/s11095-010-0358-5
State	Published - Jun 2011

Bibliographical note

Funding Information:
This work was supported by the National Science Foundation through a CAREER Award to JPF (#0448684) as well as the Maryland NanoCenter and the NispLab. The NispLab is supported in part by the NSF and as a MRSEC Shared Experimental Facility. The authors would like to thank Dr. Sameer Shah for the Lipofectamine 2000 transfection reagent and Dr. Adam Hsieh for providing ampicillin, both whom are at the University of Maryland. We would also like to thank Chen-Yu Tsao, Chi-Wei Hung and Hsuan-Chen Wu from the lab of Dr. William Bentley at University of Maryland for their contributions.

Funding

This work was supported by the National Science Foundation through a CAREER Award to JPF (#0448684) as well as the Maryland NanoCenter and the NispLab. The NispLab is supported in part by the NSF and as a MRSEC Shared Experimental Facility. The authors would like to thank Dr. Sameer Shah for the Lipofectamine 2000 transfection reagent and Dr. Adam Hsieh for providing ampicillin, both whom are at the University of Maryland. We would also like to thank Chen-Yu Tsao, Chi-Wei Hung and Hsuan-Chen Wu from the lab of Dr. William Bentley at University of Maryland for their contributions.

Funders	Funder number
University of Maryland, The Maryland NanoCenter
National Science Foundation (NSF)	0448684
Materials Research Science and Engineering Center, Harvard University

Keywords

IGF-1
cyclic acetal
gene therapy
synthetic meshes

ASJC Scopus subject areas

Biotechnology
Molecular Medicine
Pharmacology
Pharmaceutical Science
Organic Chemistry
Pharmacology (medical)

Access to Document

10.1007/s11095-010-0358-5

Cite this

@article{ff9a23f3538346dfab3ac08f4aa51a9b,

title = "Porous EH and EH-PEG scaffolds as gene delivery vehicles to skeletal muscle",

abstract = "Purpose: Synthetic biomaterials are widely used in an attempt to control the cellular behavior of regenerative tissues. This can be done by altering the chemical and physical properties of the polymeric scaffold to guide tissue repair. This paper addresses the use of a polymeric scaffold (EH network) made from the cyclic acetal monomer, 5-ethyl-5-(hydroxymethyl)-β,β- dimethyl-1,3-dioxane-2-ethanol diacrylate (EHD), as a release device for a therapeutic plasmid encoding for an insulin-like growth factor-1 green fluorescent protein fusion protein (IGF-1 GFP). Methods: Scaffolds were designed to have different porous architectures, and the impact of these architectures on plasmid release was determined. We hypothesized that IGF-1 could be delivered more effectively using a porous scaffold to allow for the release of IGF-1. Results: We showed that by altering the number of pores exposed to the surface of the network, faster plasmid loading and release were achieved. In addition, the IGF-1 GFP plasmids were found to be effective in producing IGF-1 and GFP within human skeletal muscle myoblast cell cultures. Conclusions: This work aims to show the utility of EH biomaterials for plasmid delivery for potentially localized skeletal muscle regeneration.",

keywords = "IGF-1, cyclic acetal, gene therapy, synthetic meshes",

author = "Falco, {Erin E.} and Wang, {Martha O.} and Thompson, {Joshua A.} and Chetta, {Joshua M.} and Yoon, {Diana M.} and Li, {Erik Z.} and Kulkami, {Mangesh M.} and Sameer Shah and Abhay Pandit and Roth, {J. Scott} and Fisher, {John P.}",

note = "Funding Information: This work was supported by the National Science Foundation through a CAREER Award to JPF (#0448684) as well as the Maryland NanoCenter and the NispLab. The NispLab is supported in part by the NSF and as a MRSEC Shared Experimental Facility. The authors would like to thank Dr. Sameer Shah for the Lipofectamine 2000 transfection reagent and Dr. Adam Hsieh for providing ampicillin, both whom are at the University of Maryland. We would also like to thank Chen-Yu Tsao, Chi-Wei Hung and Hsuan-Chen Wu from the lab of Dr. William Bentley at University of Maryland for their contributions.",

year = "2011",

month = jun,

doi = "10.1007/s11095-010-0358-5",

language = "English",

volume = "28",

pages = "1306--1316",

number = "6",

}

TY - JOUR

T1 - Porous EH and EH-PEG scaffolds as gene delivery vehicles to skeletal muscle

AU - Falco, Erin E.

AU - Wang, Martha O.

AU - Thompson, Joshua A.

AU - Chetta, Joshua M.

AU - Yoon, Diana M.

AU - Li, Erik Z.

AU - Kulkami, Mangesh M.

AU - Shah, Sameer

AU - Pandit, Abhay

AU - Roth, J. Scott

AU - Fisher, John P.

N1 - Funding Information: This work was supported by the National Science Foundation through a CAREER Award to JPF (#0448684) as well as the Maryland NanoCenter and the NispLab. The NispLab is supported in part by the NSF and as a MRSEC Shared Experimental Facility. The authors would like to thank Dr. Sameer Shah for the Lipofectamine 2000 transfection reagent and Dr. Adam Hsieh for providing ampicillin, both whom are at the University of Maryland. We would also like to thank Chen-Yu Tsao, Chi-Wei Hung and Hsuan-Chen Wu from the lab of Dr. William Bentley at University of Maryland for their contributions.

PY - 2011/6

Y1 - 2011/6

N2 - Purpose: Synthetic biomaterials are widely used in an attempt to control the cellular behavior of regenerative tissues. This can be done by altering the chemical and physical properties of the polymeric scaffold to guide tissue repair. This paper addresses the use of a polymeric scaffold (EH network) made from the cyclic acetal monomer, 5-ethyl-5-(hydroxymethyl)-β,β- dimethyl-1,3-dioxane-2-ethanol diacrylate (EHD), as a release device for a therapeutic plasmid encoding for an insulin-like growth factor-1 green fluorescent protein fusion protein (IGF-1 GFP). Methods: Scaffolds were designed to have different porous architectures, and the impact of these architectures on plasmid release was determined. We hypothesized that IGF-1 could be delivered more effectively using a porous scaffold to allow for the release of IGF-1. Results: We showed that by altering the number of pores exposed to the surface of the network, faster plasmid loading and release were achieved. In addition, the IGF-1 GFP plasmids were found to be effective in producing IGF-1 and GFP within human skeletal muscle myoblast cell cultures. Conclusions: This work aims to show the utility of EH biomaterials for plasmid delivery for potentially localized skeletal muscle regeneration.

AB - Purpose: Synthetic biomaterials are widely used in an attempt to control the cellular behavior of regenerative tissues. This can be done by altering the chemical and physical properties of the polymeric scaffold to guide tissue repair. This paper addresses the use of a polymeric scaffold (EH network) made from the cyclic acetal monomer, 5-ethyl-5-(hydroxymethyl)-β,β- dimethyl-1,3-dioxane-2-ethanol diacrylate (EHD), as a release device for a therapeutic plasmid encoding for an insulin-like growth factor-1 green fluorescent protein fusion protein (IGF-1 GFP). Methods: Scaffolds were designed to have different porous architectures, and the impact of these architectures on plasmid release was determined. We hypothesized that IGF-1 could be delivered more effectively using a porous scaffold to allow for the release of IGF-1. Results: We showed that by altering the number of pores exposed to the surface of the network, faster plasmid loading and release were achieved. In addition, the IGF-1 GFP plasmids were found to be effective in producing IGF-1 and GFP within human skeletal muscle myoblast cell cultures. Conclusions: This work aims to show the utility of EH biomaterials for plasmid delivery for potentially localized skeletal muscle regeneration.

KW - IGF-1

KW - cyclic acetal

KW - gene therapy

KW - synthetic meshes

UR - http://www.scopus.com/inward/record.url?scp=79959877733&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79959877733&partnerID=8YFLogxK

U2 - 10.1007/s11095-010-0358-5

DO - 10.1007/s11095-010-0358-5

M3 - Article

C2 - 21246395

AN - SCOPUS:79959877733

SN - 0724-8741

VL - 28

SP - 1306

EP - 1316

JO - Pharmaceutical Research

JF - Pharmaceutical Research

IS - 6

ER -

Porous EH and EH-PEG scaffolds as gene delivery vehicles to skeletal muscle

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this