Positive selection of EqCD8+ precursors increases equine lymphokine-activated killing

D. P. Lunn; B. R. Schram; Karen E. Vagnoni; C. S. Schobert; D. W. Horohov; O. J. Ginther

doi:10.1016/0165-2427(96)05554-7

Positive selection of EqCD8⁺ precursors increases equine lymphokine-activated killing

D. P. Lunn, B. R. Schram, Karen E. Vagnoni, C. S. Schobert, D. W. Horohov, O. J. Ginther

Veterinary Science

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

Lymphokine activated killing (LAK) is an example of natural cytotoxicity, and as such is a critical means of defense against diseases such as viral infection and neoplasia. Despite this important role, the specific molecular interactions involved in LAK or other forms of natural cytotoxicity are only partially understood. In some species, cells capable of mediating natural cytotoxicity express the CD8 molecule, although no specific role has been demonstrated for CD8 in non-MHC restricted cytotoxicity. In this study the role of the EqCD8 equine homolog of CD8 in LAK cell activity was examined. A series of LAK assays were performed using equine lymphocyte populations enriched or depleted for EqCD8 expression by positive or negative selection, The results indicate that positive selection of LAK precursors using an anti-EqCD8 (CVS8) antibody greatly increases LAK cytotoxicity, The implications for the role of the EqCD8 molecules in LAK are discussed.

Original language	English
Pages (from-to)	1-13
Number of pages	13
Journal	Veterinary Immunology and Immunopathology
Volume	53
Issue number	1-2
DOIs	https://doi.org/10.1016/0165-2427(96)05554-7
State	Published - Sep 1996

Bibliographical note

Funding Information:
We thank J.L. Stott and P.F. Moore (University of California, Davis, CA), M.A. Holmes (University of Cambridge, UK), and Julia H. Kydd (Animal Health Trust, UK) for their generousp rovision of antibodies. This researchw as supportedb y grants from the American Quarter Horse Association, and the Companion Animal Fund of the School of Veterinary Medicine, University of Wisconsin-Madison.

Keywords

CD8
Cytotoxicity
Horses
Natural killing

ASJC Scopus subject areas

Immunology
General Veterinary

Access to Document

10.1016/0165-2427(96)05554-7

Cite this

@article{534f43cb0cc149f4b1c11251bdf1ec72,

title = "Positive selection of EqCD8+ precursors increases equine lymphokine-activated killing",

abstract = "Lymphokine activated killing (LAK) is an example of natural cytotoxicity, and as such is a critical means of defense against diseases such as viral infection and neoplasia. Despite this important role, the specific molecular interactions involved in LAK or other forms of natural cytotoxicity are only partially understood. In some species, cells capable of mediating natural cytotoxicity express the CD8 molecule, although no specific role has been demonstrated for CD8 in non-MHC restricted cytotoxicity. In this study the role of the EqCD8 equine homolog of CD8 in LAK cell activity was examined. A series of LAK assays were performed using equine lymphocyte populations enriched or depleted for EqCD8 expression by positive or negative selection, The results indicate that positive selection of LAK precursors using an anti-EqCD8 (CVS8) antibody greatly increases LAK cytotoxicity, The implications for the role of the EqCD8 molecules in LAK are discussed.",

keywords = "CD8, Cytotoxicity, Horses, Natural killing",

author = "Lunn, {D. P.} and Schram, {B. R.} and Vagnoni, {Karen E.} and Schobert, {C. S.} and Horohov, {D. W.} and Ginther, {O. J.}",

note = "Funding Information: We thank J.L. Stott and P.F. Moore (University of California, Davis, CA), M.A. Holmes (University of Cambridge, UK), and Julia H. Kydd (Animal Health Trust, UK) for their generousp rovision of antibodies. This researchw as supportedb y grants from the American Quarter Horse Association, and the Companion Animal Fund of the School of Veterinary Medicine, University of Wisconsin-Madison.",

year = "1996",

month = sep,

doi = "10.1016/0165-2427(96)05554-7",

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T1 - Positive selection of EqCD8+ precursors increases equine lymphokine-activated killing

AU - Lunn, D. P.

AU - Schram, B. R.

AU - Vagnoni, Karen E.

AU - Schobert, C. S.

AU - Horohov, D. W.

AU - Ginther, O. J.

N1 - Funding Information: We thank J.L. Stott and P.F. Moore (University of California, Davis, CA), M.A. Holmes (University of Cambridge, UK), and Julia H. Kydd (Animal Health Trust, UK) for their generousp rovision of antibodies. This researchw as supportedb y grants from the American Quarter Horse Association, and the Companion Animal Fund of the School of Veterinary Medicine, University of Wisconsin-Madison.

PY - 1996/9

Y1 - 1996/9

N2 - Lymphokine activated killing (LAK) is an example of natural cytotoxicity, and as such is a critical means of defense against diseases such as viral infection and neoplasia. Despite this important role, the specific molecular interactions involved in LAK or other forms of natural cytotoxicity are only partially understood. In some species, cells capable of mediating natural cytotoxicity express the CD8 molecule, although no specific role has been demonstrated for CD8 in non-MHC restricted cytotoxicity. In this study the role of the EqCD8 equine homolog of CD8 in LAK cell activity was examined. A series of LAK assays were performed using equine lymphocyte populations enriched or depleted for EqCD8 expression by positive or negative selection, The results indicate that positive selection of LAK precursors using an anti-EqCD8 (CVS8) antibody greatly increases LAK cytotoxicity, The implications for the role of the EqCD8 molecules in LAK are discussed.

AB - Lymphokine activated killing (LAK) is an example of natural cytotoxicity, and as such is a critical means of defense against diseases such as viral infection and neoplasia. Despite this important role, the specific molecular interactions involved in LAK or other forms of natural cytotoxicity are only partially understood. In some species, cells capable of mediating natural cytotoxicity express the CD8 molecule, although no specific role has been demonstrated for CD8 in non-MHC restricted cytotoxicity. In this study the role of the EqCD8 equine homolog of CD8 in LAK cell activity was examined. A series of LAK assays were performed using equine lymphocyte populations enriched or depleted for EqCD8 expression by positive or negative selection, The results indicate that positive selection of LAK precursors using an anti-EqCD8 (CVS8) antibody greatly increases LAK cytotoxicity, The implications for the role of the EqCD8 molecules in LAK are discussed.

KW - CD8

KW - Cytotoxicity

KW - Horses

KW - Natural killing

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