Potential anti-obesity effects of a long-acting cocaine hydrolase

Xirong Zheng, Jing Deng, Ting Zhang, Jianzhuang Yao, Fang Zheng, Chang Guo Zhan

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

A long-acting cocaine hydrolase, known as CocH3-Fc(M3), engineered from human butyrylcholinesterase (BChE) was tested, in this study, for its potential anti-obesity effects. Mice on a high-fat diet gained significantly less body weight when treated weekly with 1 mg/kg CocH3-Fc(M3) compared to control mice, though their food intake was similar. There is no correlation between the average body weight and the average food intake, which is consistent with the previously reported observation in BChE knockout mice. In addition, molecular modeling was carried out to understand how ghrelin binds with CocH3, showing that ghrelin binds with CocH3 in a similar mode as ghrelin binding with wild-type human BChE. The similar binding structures explains why CocH3 and BChE have similar catalytic activity against ghrelin.

Original languageEnglish
Pages (from-to)99-103
Number of pages5
JournalChemico-Biological Interactions
Volume259
DOIs
StatePublished - Nov 25 2016

Bibliographical note

Publisher Copyright:
© 2016 Elsevier Ireland Ltd

Keywords

  • Appetite control
  • Cocaine hydrolase
  • Enzyme
  • Hormone
  • Obesity

ASJC Scopus subject areas

  • Toxicology

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