TY - JOUR
T1 - Potential anti-obesity effects of a long-acting cocaine hydrolase
AU - Zheng, Xirong
AU - Deng, Jing
AU - Zhang, Ting
AU - Yao, Jianzhuang
AU - Zheng, Fang
AU - Zhan, Chang Guo
N1 - Publisher Copyright:
© 2016 Elsevier Ireland Ltd
PY - 2016/11/25
Y1 - 2016/11/25
N2 - A long-acting cocaine hydrolase, known as CocH3-Fc(M3), engineered from human butyrylcholinesterase (BChE) was tested, in this study, for its potential anti-obesity effects. Mice on a high-fat diet gained significantly less body weight when treated weekly with 1 mg/kg CocH3-Fc(M3) compared to control mice, though their food intake was similar. There is no correlation between the average body weight and the average food intake, which is consistent with the previously reported observation in BChE knockout mice. In addition, molecular modeling was carried out to understand how ghrelin binds with CocH3, showing that ghrelin binds with CocH3 in a similar mode as ghrelin binding with wild-type human BChE. The similar binding structures explains why CocH3 and BChE have similar catalytic activity against ghrelin.
AB - A long-acting cocaine hydrolase, known as CocH3-Fc(M3), engineered from human butyrylcholinesterase (BChE) was tested, in this study, for its potential anti-obesity effects. Mice on a high-fat diet gained significantly less body weight when treated weekly with 1 mg/kg CocH3-Fc(M3) compared to control mice, though their food intake was similar. There is no correlation between the average body weight and the average food intake, which is consistent with the previously reported observation in BChE knockout mice. In addition, molecular modeling was carried out to understand how ghrelin binds with CocH3, showing that ghrelin binds with CocH3 in a similar mode as ghrelin binding with wild-type human BChE. The similar binding structures explains why CocH3 and BChE have similar catalytic activity against ghrelin.
KW - Appetite control
KW - Cocaine hydrolase
KW - Enzyme
KW - Hormone
KW - Obesity
UR - http://www.scopus.com/inward/record.url?scp=84966714087&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84966714087&partnerID=8YFLogxK
U2 - 10.1016/j.cbi.2016.05.010
DO - 10.1016/j.cbi.2016.05.010
M3 - Article
C2 - 27163854
AN - SCOPUS:84966714087
SN - 0009-2797
VL - 259
SP - 99
EP - 103
JO - Chemico-Biological Interactions
JF - Chemico-Biological Interactions
ER -