Potential for bacteriophage cocktail to complement commercial sanitizer use on produce against escherichia coli o157:H7

Badrinath Vengarai Jagannathan, Steven Kitchens, Paul Priyesh Vijayakumar, Stuart Price, Melissa Morgan

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

The increasing concern for food safety has created a need to evaluate novel techniques to eliminate or control pathogens, resulting in safe food. In this study, four bacteriophages of bovine origin, specific to E. coli O157:H7, were successfully isolated and characterized. A microplate reader assay demonstrated the efficacy of the bacteriophage (phage) cocktail against E. coli O157:H7 resulting in a significant reduction (p < 0.01) in the target pathogen population. The phage cocktail demonstrated significant efficacy (p <0.05) against E. coli O157:H7 in the presence of the most utilized sanitizers in the United States, namely 100 parts per million (ppm) free chlorine and 100-ppm peroxyacetic acid. Survival in the sanitizer concentrations demonstrates the potential use of phage cocktail and sanitizer synergistically to enhance sanitation operations in the food industry.

Original languageEnglish
Article number1316
Pages (from-to)1-13
Number of pages13
JournalMicroorganisms
Volume8
Issue number9
DOIs
StatePublished - Sep 2020

Bibliographical note

Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.

Keywords

  • Bacteriophage
  • Characterization
  • E. coli
  • Microplate
  • Sanitizer

ASJC Scopus subject areas

  • Microbiology
  • Virology
  • Microbiology (medical)

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