Abstract
The increasing concern for food safety has created a need to evaluate novel techniques to eliminate or control pathogens, resulting in safe food. In this study, four bacteriophages of bovine origin, specific to E. coli O157:H7, were successfully isolated and characterized. A microplate reader assay demonstrated the efficacy of the bacteriophage (phage) cocktail against E. coli O157:H7 resulting in a significant reduction (p < 0.01) in the target pathogen population. The phage cocktail demonstrated significant efficacy (p <0.05) against E. coli O157:H7 in the presence of the most utilized sanitizers in the United States, namely 100 parts per million (ppm) free chlorine and 100-ppm peroxyacetic acid. Survival in the sanitizer concentrations demonstrates the potential use of phage cocktail and sanitizer synergistically to enhance sanitation operations in the food industry.
| Original language | English |
|---|---|
| Article number | 1316 |
| Pages (from-to) | 1-13 |
| Number of pages | 13 |
| Journal | Microorganisms |
| Volume | 8 |
| Issue number | 9 |
| DOIs | |
| State | Published - Sep 2020 |
Bibliographical note
Publisher Copyright:© 2020 by the authors. Licensee MDPI, Basel, Switzerland.
Funding
Funding: This work was supported by the Food Safety Outreach Competitive Grants Program, Grant No. 2017-70020-27246 from the USDA National Institute of Food and Agriculture.
| Funders |
|---|
| US Department of Agriculture National Institute of Food and Agriculture, Agriculture and Food Research Initiative |
Keywords
- Bacteriophage
- Characterization
- E. coli
- Microplate
- Sanitizer
ASJC Scopus subject areas
- Microbiology
- Microbiology (medical)
- Virology