PPAR-γ agonist protects against intestinal injury during necrotizing enterocolitis

Naira Baregamian, Joshua M. Mourot, Amie R. Ballard, B. Mark Evers, Dai H. Chung

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

Necrotizing enterocolitis (NEC) remains a lethal condition for many premature infants. Peroxisome proliferator-activated receptor-γ (PPAR-γ), a member of the nuclear hormone receptor family, has been shown to play a protective role in cellular inflammatory responses; however, its role in NEC is not clearly defined. We sought to examine the expression of PPAR-γ in the intestine using an ischemia-reperfusion (I/R) model of NEC, and to assess whether PPAR-γ agonist treatment would ameliorate I/R-induced gut injury. Swiss-Webster mice were randomized to receive sham (control) or I/R injury to the gut induced by transient occlusion of superior mesenteric artery for 45 min with variable periods of reperfusion. I/R injury resulted in early induction of PPAR-γ expression and activation of NF-κB in small intestine. Pretreatment with PPAR-γ agonist, 15d-PGJ2, attenuated intestinal NF-κB response and I/R-induced gut injury. Activation of PPAR-γ demonstrated a protective effect on small bowel during I/R-induced gut injury.

Original languageEnglish
Pages (from-to)423-427
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume379
Issue number2
DOIs
StatePublished - Feb 6 2009

Bibliographical note

Funding Information:
The authors thank Karen Martin for paper preparation. This work was supported by Grants, R01DK48498, R01DK61470, PO1DK35608 and T32DK07639, from the National Institutes of Health and by the Shriners Burns Hospital Grant (No. 8580).

Keywords

  • Ischemia-reperfusion injury
  • Necrotizing enterocolitis
  • Nuclear factor-kappaB
  • Peroxisome proliferator-activated receptor-γ

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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