PPAR-γ agonist protects against intestinal injury during necrotizing enterocolitis

Naira Baregamian; Joshua M. Mourot; Amie R. Ballard; B. Mark Evers; Dai H. Chung

doi:10.1016/j.bbrc.2008.11.155

PPAR-γ agonist protects against intestinal injury during necrotizing enterocolitis

Naira Baregamian, Joshua M. Mourot, Amie R. Ballard, B. Mark Evers, Dai H. Chung

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

Necrotizing enterocolitis (NEC) remains a lethal condition for many premature infants. Peroxisome proliferator-activated receptor-γ (PPAR-γ), a member of the nuclear hormone receptor family, has been shown to play a protective role in cellular inflammatory responses; however, its role in NEC is not clearly defined. We sought to examine the expression of PPAR-γ in the intestine using an ischemia-reperfusion (I/R) model of NEC, and to assess whether PPAR-γ agonist treatment would ameliorate I/R-induced gut injury. Swiss-Webster mice were randomized to receive sham (control) or I/R injury to the gut induced by transient occlusion of superior mesenteric artery for 45 min with variable periods of reperfusion. I/R injury resulted in early induction of PPAR-γ expression and activation of NF-κB in small intestine. Pretreatment with PPAR-γ agonist, 15d-PGJ₂, attenuated intestinal NF-κB response and I/R-induced gut injury. Activation of PPAR-γ demonstrated a protective effect on small bowel during I/R-induced gut injury.

Original language	English
Pages (from-to)	423-427
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	379
Issue number	2
DOIs	https://doi.org/10.1016/j.bbrc.2008.11.155
State	Published - Feb 6 2009

Bibliographical note

Funding Information:
The authors thank Karen Martin for paper preparation. This work was supported by Grants, R01DK48498, R01DK61470, PO1DK35608 and T32DK07639, from the National Institutes of Health and by the Shriners Burns Hospital Grant (No. 8580).

Keywords

Ischemia-reperfusion injury
Necrotizing enterocolitis
Nuclear factor-kappaB
Peroxisome proliferator-activated receptor-γ

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "PPAR-γ agonist protects against intestinal injury during necrotizing enterocolitis",

abstract = "Necrotizing enterocolitis (NEC) remains a lethal condition for many premature infants. Peroxisome proliferator-activated receptor-γ (PPAR-γ), a member of the nuclear hormone receptor family, has been shown to play a protective role in cellular inflammatory responses; however, its role in NEC is not clearly defined. We sought to examine the expression of PPAR-γ in the intestine using an ischemia-reperfusion (I/R) model of NEC, and to assess whether PPAR-γ agonist treatment would ameliorate I/R-induced gut injury. Swiss-Webster mice were randomized to receive sham (control) or I/R injury to the gut induced by transient occlusion of superior mesenteric artery for 45 min with variable periods of reperfusion. I/R injury resulted in early induction of PPAR-γ expression and activation of NF-κB in small intestine. Pretreatment with PPAR-γ agonist, 15d-PGJ2, attenuated intestinal NF-κB response and I/R-induced gut injury. Activation of PPAR-γ demonstrated a protective effect on small bowel during I/R-induced gut injury.",

keywords = "Ischemia-reperfusion injury, Necrotizing enterocolitis, Nuclear factor-kappaB, Peroxisome proliferator-activated receptor-γ",

author = "Naira Baregamian and Mourot, {Joshua M.} and Ballard, {Amie R.} and Evers, {B. Mark} and Chung, {Dai H.}",

note = "Funding Information: The authors thank Karen Martin for paper preparation. This work was supported by Grants, R01DK48498, R01DK61470, PO1DK35608 and T32DK07639, from the National Institutes of Health and by the Shriners Burns Hospital Grant (No. 8580).",

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AU - Ballard, Amie R.

AU - Evers, B. Mark

AU - Chung, Dai H.

N1 - Funding Information: The authors thank Karen Martin for paper preparation. This work was supported by Grants, R01DK48498, R01DK61470, PO1DK35608 and T32DK07639, from the National Institutes of Health and by the Shriners Burns Hospital Grant (No. 8580).

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N2 - Necrotizing enterocolitis (NEC) remains a lethal condition for many premature infants. Peroxisome proliferator-activated receptor-γ (PPAR-γ), a member of the nuclear hormone receptor family, has been shown to play a protective role in cellular inflammatory responses; however, its role in NEC is not clearly defined. We sought to examine the expression of PPAR-γ in the intestine using an ischemia-reperfusion (I/R) model of NEC, and to assess whether PPAR-γ agonist treatment would ameliorate I/R-induced gut injury. Swiss-Webster mice were randomized to receive sham (control) or I/R injury to the gut induced by transient occlusion of superior mesenteric artery for 45 min with variable periods of reperfusion. I/R injury resulted in early induction of PPAR-γ expression and activation of NF-κB in small intestine. Pretreatment with PPAR-γ agonist, 15d-PGJ2, attenuated intestinal NF-κB response and I/R-induced gut injury. Activation of PPAR-γ demonstrated a protective effect on small bowel during I/R-induced gut injury.

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PPAR-γ agonist protects against intestinal injury during necrotizing enterocolitis

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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