Practical survey on antibiotic-resistant bacterial communities in livestock manure and manure-amended soil

Qingxiang Yang, Ruifei Wang, Siwei Ren, Marton Szoboszlay, Luke A. Moe

Research output: Contribution to journalArticlepeer-review

33 Scopus citations

Abstract

Through livestock manure fertilization, antibiotics, antibiotic-resistant bacteria and genes are transferred to agricultural soils, resulting in a high prevalence of antibiotic-resistant bacteria in the soil. It is not clear, however, whether a correlation exists between resistant bacterial populations in manure and manure-amended soil. In this work, we demonstrate that the prevalence of cephalexin-, amoxicillin-, kanamycin- and gentamicin-resistant bacteria as well as bacteria simultaneously resistant to all four antibiotics was much higher in manure-amended soils than in manure-free soil. 454-pyrosequencing indicated that the ARB and multiple antibiotic-resistant bacteria (MARB) in swine or chicken manure and manure-amended soil were mainly distributed among Sphingobacterium, Myroides, Enterococcus, Comamonas and unclassified Flavobacteriaceae. The genus Sphingobacterium was highly prevalent among ARB from swine manure and manure-amended soil, and was also the most dominant genus among MARB from chicken manure and manure-amended soil. Other dominant genera among ARB or MARB populations in manure samples, including Myroides, Enterococcus and Comamonas, could not be detected or were detected at very low relative abundance in manure-amended soil. The present study suggests the possibility of transfer of ARBs from livestock manures to soils and persistence of ARB in these environments.

Original languageEnglish
Pages (from-to)14-23
Number of pages10
JournalJournal of Environmental Science and Health - Part B Pesticides, Food Contaminants, and Agricultural Wastes
Volume51
Issue number1
DOIs
StatePublished - Jan 2 2016

Bibliographical note

Publisher Copyright:
© 2016 Taylor & Francis Group, LLC.

Keywords

  • 454-pyrosequencing
  • antibiotic resistance
  • antibiotic resistant bacteria
  • bacterial community
  • livestock manure
  • manure-amended soil

ASJC Scopus subject areas

  • Food Science
  • Pollution

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