PRC1 associates with the hsp70i promoter and interacts with HSF2 during mitosis

Lynea A. Murphy, Donald C. Wilkerson, Yiling Hong, Kevin D. Sarge

Research output: Contribution to journalArticlepeer-review

8 Scopus citations


Mitosis is a series of events leading to division of a cell by the process known as cytokinesis. Protein regulating cytokinesis 1 (PRC1) is a CDK substrate that associates with the mitotic spindle and functions in microtubule bundling. Previous studies revealed that loss of PRC1 is associated with chromosomal mis-segregation and atypical chromosome alignment. HSF2 is a DNA binding protein that we previously showed bookmarks the hsp70i gene during mitosis, an epigenetic mechanism which allows the hsp70i gene to re-establish transcriptional competence early in G1. Another study demonstrated that HSF2-/- mouse embryonic fibroblasts (MEFs) exhibit increased numbers of multinucleated cells vs. wild-type MEFs. This suggests that HSF2 is important for proper cytokinesis, but the mechanism was unknown. Here we report the existence of a direct interaction between HSF2 and PRC1. HSF2 and PRC1 associate during mitosis and co-localize during this phase of the cell cycle. PRC1 does not interact with the related protein HSF1, indicating the specificity of the HSF2-PRC1 interaction. Intriguingly, PRC1 is associated with the hsp70i promoter during mitosis. These results provide a potential mechanistic basis for the defective cytokinesis phenotype exhibited by HSF2-/- cells, as well as suggest a potential role for PRC1 in HSF2-mediated gene bookmarking.

Original languageEnglish
Pages (from-to)2224-2230
Number of pages7
JournalExperimental Cell Research
Issue number11-12
StatePublished - 2008

Bibliographical note

Funding Information:
This research was supported by NIH grants GM61053 and GM64606 to K.D.S., Training Grant T32 ES-07266 (Department of Toxicology, P.I.: Dr. Mary Vore) support for L.A.M., and NIH postdoctoral support (HD50043) to D.C.W.


  • HSF2
  • PRC1
  • bookmarking
  • cytokinesis
  • hsp70i gene

ASJC Scopus subject areas

  • Cell Biology


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