Preconditioning Layers Affect Osteoblastic Cell Adhesion to Orthopedic Implant Surfaces

James D. Boyd, Martha E. Grady

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review


Bacteria can proliferate orthopedic implants, resulting in infection rates as high as 5%. A consistent problem across implantology is the development of surfaces which successfully promote the adhesion and propagation of healthy fibroblast and osteoblast cells while deterring formation of bacterial biofilms. Selecting surface configurations which favor cell adhesion will lead to decreased infection rates. Progress in identifying appropriate surface configurations is hindered by the lack of quantitative adhesion techniques capable of comparing adhesion of cells and biofilms directly. Recent advancements in adhesion techniques have allowed for quantitatively measured adhesion strengths of both bacterial biofilms and cell monolayers using the laser spallation technique. The quantified stress-based adhesion values allow surface and environmental factors that modulate both bacterial and cell adhesion to implant surfaces to be evaluated. During implantation, blood propagates wound sites completely coating implant surfaces. Quantitatively determining the impact of preconditioning layers that accumulate on the implant surface on cell adhesion is vital to predict implant behavior. Previous work has demonstrated that these preconditioning layers either negatively or neutrally impact bacterial adhesion to titanium implant surfaces. This study focuses on the impact that blood plasma and fibronectin coatings have on the adhesion of osteoblastic (MG 63) cells and fibroblasts to the same titanium surfaces. Adhesion results indicate that preconditioning layers and increased surface roughness positively impact cell adhesion. Incorporating the increased adhesion values for cell adhesion into the Adhesion Index demonstrates that increased surface roughness, coupled with natural wound healing preconditioning of surfaces, yields positive biocompatibility.

Original languageEnglish
Title of host publicationMechanics of Composite, Hybrid and Multi-functional Materials, Volume 5 - Proceedings of the 2022 Annual Conference on Experimental and Applied Mechanics
EditorsVijaya Chalivendra, Frank Gardea
Number of pages3
StatePublished - 2023
EventSEM Annual Conference and Exposition on Experimental and Applied Mechanics, 2022 - Pittsburgh, United States
Duration: Jun 13 2022Jun 16 2022

Publication series

NameConference Proceedings of the Society for Experimental Mechanics Series
ISSN (Print)2191-5644
ISSN (Electronic)2191-5652


ConferenceSEM Annual Conference and Exposition on Experimental and Applied Mechanics, 2022
Country/TerritoryUnited States

Bibliographical note

Publisher Copyright:
© 2023, The Society for Experimental Mechanics, Inc.


  • Adhesion
  • Biofilms
  • Blood plasma
  • Laser spallation
  • MG 63
  • Surface treatment

ASJC Scopus subject areas

  • General Engineering
  • Computational Mechanics
  • Mechanical Engineering


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