Preferential activation of [3H]phorbol-12,13-dibutyrate binding by AMPA (α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid) in neonatal striatal cell cultures

Michael McMillian, J. S. Hong, Keith R. Pennypacker

Research output: Contribution to journalArticlepeer-review

10 Scopus citations


Activation of excitatory amino acid receptors increased [3H]phorbol-12,13-dibutyrate ([3H]PdBu) binding in four week cultures of striatal cells from postnatal day 7 rat pups (PN7), and in PN7 cells co-cultured the fourth week with striatal cells from postnatal day 1 rat pups. Kainate (KA), trans-1-amino-cyclopentyl-1,3-dicarboxylate (ACPD), and N-methyl-d-aspartate (NMDA) increased [3H]PdBu binding equally in both types of cultures, but α-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid (AMPA) increased binding by 3-fold in the co-cultures. Thus, [3H]PdBu binding in these two types of striatal cultures offers a simple model system for studying the regulation of AMPA/KA receptor responses.

Original languageEnglish
Pages (from-to)307-310
Number of pages4
JournalBrain Research
Issue number2
StatePublished - Oct 16 1992


  • AMPA
  • Excitatory amino acid receptor
  • Glutamate
  • Kainate
  • Phorbol 12,13-dibutyrate
  • Striatal

ASJC Scopus subject areas

  • Neuroscience (all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology


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