TY - JOUR
T1 - Preparative purification of Escherichia coli heat-stable enterotoxin
AU - Saeed, Abdul M.K.
AU - Greenberg, Richard N.
PY - 1985/12
Y1 - 1985/12
N2 - Heat-stable enterotoxin (STa) isolated from bovine Escherichia coli strains was purified to homogeneity by growing the bacterial strains in a chemically defined medium, desalting, and concentrating the culture filtrate by batch adsorption chromatography on Amberlite XAD-2 resin, batch adsorption chromatography on reversed-phase silica, and preparative reversed-phase high-performance liquid chromatography. This rapid preparative purification scheme gave high recovery yields of pure STa which exhibited biochemical homology to STa purified by more complicated procedures.
AB - Heat-stable enterotoxin (STa) isolated from bovine Escherichia coli strains was purified to homogeneity by growing the bacterial strains in a chemically defined medium, desalting, and concentrating the culture filtrate by batch adsorption chromatography on Amberlite XAD-2 resin, batch adsorption chromatography on reversed-phase silica, and preparative reversed-phase high-performance liquid chromatography. This rapid preparative purification scheme gave high recovery yields of pure STa which exhibited biochemical homology to STa purified by more complicated procedures.
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U2 - 10.1016/0003-2697(85)90200-3
DO - 10.1016/0003-2697(85)90200-3
M3 - Article
C2 - 3913332
AN - SCOPUS:0022312731
SN - 0003-2697
VL - 151
SP - 431
EP - 437
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 2
ER -