Preparative purification of Escherichia coli heat-stable enterotoxin

Abdul M.K. Saeed, Richard N. Greenberg

Research output: Contribution to journalArticlepeer-review

17 Scopus citations


Heat-stable enterotoxin (STa) isolated from bovine Escherichia coli strains was purified to homogeneity by growing the bacterial strains in a chemically defined medium, desalting, and concentrating the culture filtrate by batch adsorption chromatography on Amberlite XAD-2 resin, batch adsorption chromatography on reversed-phase silica, and preparative reversed-phase high-performance liquid chromatography. This rapid preparative purification scheme gave high recovery yields of pure STa which exhibited biochemical homology to STa purified by more complicated procedures.

Original languageEnglish
Pages (from-to)431-437
Number of pages7
JournalAnalytical Biochemistry
Issue number2
StatePublished - Dec 1985

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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