TY - JOUR
T1 - Presence of erythroglycan on human K-562 chronic myelogenous leukemia-derived cells
AU - Turco, S. J.
AU - Rush, J. S.
AU - Laine, R. A.
N1 - Copyright:
Copyright 2004 Elsevier B.V., All rights reserved.
PY - 1980
Y1 - 1980
N2 - Total glycopeptides from human K-562 cells, labeled metabolically with [3H]glucosamine or [3H]mannose, were prepared by extracting the cells with organic solvents to remove lipids and by digesting the residue with pronase. 3H-labeled glycopeptides were fractionated on Sephadex G-50 revealing a high molecular weight fraction (M(r) = 7,000 to 11,000), comprising approximately 10% of the [3H]glucosamine and 25% of the [3H]mannose label. Digestion of this glycopeptide fraction with endo-β-galactosidase from Escherichia freundii, specific for a repeating structure of Gal(β1 → 4)GlcNAc(β1→3), results in the following four products as resolved by Bio-Gel P-2 gel filtration: 1) a disaccharide with the structure β-2-deoxy-2-acetamidoglucosyl → β-galactose; 2) a trisaccharide with the structure β-galactosyl → β-2-deoxy-2-acetamidoglucosyl → β-galactose; 3) a tetrasaccharide with the sequence α-N-acetylneuraminyl → β-galactosyl → β-2-deoxy-2-acetamidoglucosyl → β-galactose; and 4) a larger, complex fragment which contains mannose and β-2-deoxy-2-acetamidoglucose and which is probably the protein linkage region. In addition, visualization of radiolabeled glycoproteins by fluorography on polyacrylamide gels revealed a 105,000-dalton 'Band 3'-like glycoprotein and other bands that were sensitive to endo-β-galactosidase. These results indicate that the K-562 cell line bears a glycopeptide, erythroglycan, which has been found on erythrocytes, and that this polymer is expressed mainly in the fetal form as a linear chain.
AB - Total glycopeptides from human K-562 cells, labeled metabolically with [3H]glucosamine or [3H]mannose, were prepared by extracting the cells with organic solvents to remove lipids and by digesting the residue with pronase. 3H-labeled glycopeptides were fractionated on Sephadex G-50 revealing a high molecular weight fraction (M(r) = 7,000 to 11,000), comprising approximately 10% of the [3H]glucosamine and 25% of the [3H]mannose label. Digestion of this glycopeptide fraction with endo-β-galactosidase from Escherichia freundii, specific for a repeating structure of Gal(β1 → 4)GlcNAc(β1→3), results in the following four products as resolved by Bio-Gel P-2 gel filtration: 1) a disaccharide with the structure β-2-deoxy-2-acetamidoglucosyl → β-galactose; 2) a trisaccharide with the structure β-galactosyl → β-2-deoxy-2-acetamidoglucosyl → β-galactose; 3) a tetrasaccharide with the sequence α-N-acetylneuraminyl → β-galactosyl → β-2-deoxy-2-acetamidoglucosyl → β-galactose; and 4) a larger, complex fragment which contains mannose and β-2-deoxy-2-acetamidoglucose and which is probably the protein linkage region. In addition, visualization of radiolabeled glycoproteins by fluorography on polyacrylamide gels revealed a 105,000-dalton 'Band 3'-like glycoprotein and other bands that were sensitive to endo-β-galactosidase. These results indicate that the K-562 cell line bears a glycopeptide, erythroglycan, which has been found on erythrocytes, and that this polymer is expressed mainly in the fetal form as a linear chain.
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M3 - Article
C2 - 6928849
AN - SCOPUS:0019313852
SN - 0021-9258
VL - 255
SP - 3266
EP - 3269
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 8
ER -