Prevalence and characterization of seven-segmented influenza viruses in bovine respiratory disease complex

Tirth Uprety; Chithra C. Sreenivasan; Milton Thomas; Ben Hause; Jane Christopher-Hennings; Dale Miskimis; Angela Pillatzki; Eric Nelson; Dan Wang; Li Feng

doi:10.1016/j.virol.2023.109859

Prevalence and characterization of seven-segmented influenza viruses in bovine respiratory disease complex

Tirth Uprety, Chithra C. Sreenivasan, Milton Thomas, Ben Hause, Jane Christopher-Hennings, Dale Miskimis, Angela Pillatzki, Eric Nelson, Dan Wang, Li Feng

Research output: Contribution to journal › Article › peer-review

Abstract

Bovine respiratory disease (BRD) complex is a multifactorial respiratory disease of cattle. Seven-segmented influenza C (ICV) and D (IDV) viruses have been identified in cattle with BRD, however, molecular epidemiology and prevalence of IDV and ICV in the diseased population remain poorly characterized. Here, we conducted a molecular screening of 208 lung samples of bovine pneumonia cases for the presence of IDV and ICV. Our results demonstrated that both viruses were prevalent in BRD cases and the overall positivity rates of IDV and ICV were 20.88% and 5.99% respectively. Further analysis of three IDV strains isolated from lungs of cattle with BRD showed that these lung-tropic strains belonged to D/Michigan/2019 clade and diverged antigenically from the circulating dominant IDV clades D/OK and D/660. Our results reveal that IDV and ICV are associated with BRD complex and support a role for IDV and ICV in the etiology of BRD.

Original language	English
Article number	109859
Journal	Virology
Volume	587
DOIs	https://doi.org/10.1016/j.virol.2023.109859
State	Published - Oct 2023

Bibliographical note

Publisher Copyright:
© 2023 Elsevier Inc.

Funding

This study was supported by NIH R01AI141889 and the SD-CBRC supported by the State of South Dakota's Governor's Office of Economic Development . The authors also thank the support provided by the William Robert Mills Chair Endowment Fund and Agricultural Experiment Station of the University of Kentucky. The members of the Wang-Li lab are particularly appreciated for their great help in animal experiments described in this manuscript.

Funders	Funder number
SD-CBRC
National Institutes of Health (NIH)	R01AI141889
National Institutes of Health (NIH)
University of Kentucky
South Dakota Governor's Office of Economic Development

Keywords

Bovine respiratory disease
Cattle
Influenza C virus
Influenza D virus

ASJC Scopus subject areas

Virology

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10.1016/j.virol.2023.109859

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title = "Prevalence and characterization of seven-segmented influenza viruses in bovine respiratory disease complex",

abstract = "Bovine respiratory disease (BRD) complex is a multifactorial respiratory disease of cattle. Seven-segmented influenza C (ICV) and D (IDV) viruses have been identified in cattle with BRD, however, molecular epidemiology and prevalence of IDV and ICV in the diseased population remain poorly characterized. Here, we conducted a molecular screening of 208 lung samples of bovine pneumonia cases for the presence of IDV and ICV. Our results demonstrated that both viruses were prevalent in BRD cases and the overall positivity rates of IDV and ICV were 20.88% and 5.99% respectively. Further analysis of three IDV strains isolated from lungs of cattle with BRD showed that these lung-tropic strains belonged to D/Michigan/2019 clade and diverged antigenically from the circulating dominant IDV clades D/OK and D/660. Our results reveal that IDV and ICV are associated with BRD complex and support a role for IDV and ICV in the etiology of BRD.",

keywords = "Bovine respiratory disease, Cattle, Influenza C virus, Influenza D virus",

author = "Tirth Uprety and Sreenivasan, {Chithra C.} and Milton Thomas and Ben Hause and Jane Christopher-Hennings and Dale Miskimis and Angela Pillatzki and Eric Nelson and Dan Wang and Li Feng",

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month = oct,

doi = "10.1016/j.virol.2023.109859",

language = "English",

volume = "587",

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AU - Uprety, Tirth

AU - Sreenivasan, Chithra C.

AU - Thomas, Milton

AU - Hause, Ben

AU - Christopher-Hennings, Jane

AU - Miskimis, Dale

AU - Pillatzki, Angela

AU - Nelson, Eric

AU - Wang, Dan

AU - Feng, Li

PY - 2023/10

Y1 - 2023/10

N2 - Bovine respiratory disease (BRD) complex is a multifactorial respiratory disease of cattle. Seven-segmented influenza C (ICV) and D (IDV) viruses have been identified in cattle with BRD, however, molecular epidemiology and prevalence of IDV and ICV in the diseased population remain poorly characterized. Here, we conducted a molecular screening of 208 lung samples of bovine pneumonia cases for the presence of IDV and ICV. Our results demonstrated that both viruses were prevalent in BRD cases and the overall positivity rates of IDV and ICV were 20.88% and 5.99% respectively. Further analysis of three IDV strains isolated from lungs of cattle with BRD showed that these lung-tropic strains belonged to D/Michigan/2019 clade and diverged antigenically from the circulating dominant IDV clades D/OK and D/660. Our results reveal that IDV and ICV are associated with BRD complex and support a role for IDV and ICV in the etiology of BRD.

AB - Bovine respiratory disease (BRD) complex is a multifactorial respiratory disease of cattle. Seven-segmented influenza C (ICV) and D (IDV) viruses have been identified in cattle with BRD, however, molecular epidemiology and prevalence of IDV and ICV in the diseased population remain poorly characterized. Here, we conducted a molecular screening of 208 lung samples of bovine pneumonia cases for the presence of IDV and ICV. Our results demonstrated that both viruses were prevalent in BRD cases and the overall positivity rates of IDV and ICV were 20.88% and 5.99% respectively. Further analysis of three IDV strains isolated from lungs of cattle with BRD showed that these lung-tropic strains belonged to D/Michigan/2019 clade and diverged antigenically from the circulating dominant IDV clades D/OK and D/660. Our results reveal that IDV and ICV are associated with BRD complex and support a role for IDV and ICV in the etiology of BRD.

KW - Bovine respiratory disease

KW - Cattle

KW - Influenza C virus

KW - Influenza D virus

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Prevalence and characterization of seven-segmented influenza viruses in bovine respiratory disease complex

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

Access to Document

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