Prevalence of antibodies to Sarcocystis neurona and Neospora hughesi in horses from Mexico

Michelle R. Yeargan; Cosme Alvarado-Esquivel; Jitender P. Dubey; Daniel K. Howe

doi:10.1051/parasite/2013029

Prevalence of antibodies to Sarcocystis neurona and Neospora hughesi in horses from Mexico

Michelle R. Yeargan, Cosme Alvarado-Esquivel, Jitender P. Dubey, Daniel K. Howe

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

Equine protozoal myeloencephalitis (EPM) is a debilitating disease of horses caused by Sarcocystis neurona and Neospora hughesi. Sera from 495 horses in Durango State, Mexico were tested for anti-protozoal antibodies using enzyme-linked immunosorbent assays (ELISAs) based on major surface antigens of these two parasites. Antibodies to S. neurona were detected in 240 (48.5%) of the 495 horse sera tested with the rSnSAG2/4/3 trivalent ELISA. Multivariate analysis showed that exposure to S. neurona was associated with age, feeding grains and crops, and small herd size. Antibodies to N. hughesi were found in 15 (3.0%) of the 495 horse sera tested with the rNhSAG1 ELISA and confirmed by Western blot of N. hughesi tachyzoite antigen. This is the first report of S. neurona and N. hughesi exposure in horses in Mexico, and it affirms that EPM should be in the differential diagnosis for horses exhibiting signs of neurologic disease in this country.

Original language	English
Article number	29
Journal	Parasite
Volume	20
Issue number	1
DOIs	https://doi.org/10.1051/parasite/2013029
State	Published - 2013

Bibliographical note

Funding Information:
This research was funded partly by the Amerman Family Equine Research Endowment. The information reported in this paper (Manuscript #13-14-109) is part of a project of the Kentucky Agricultural Experiment Station and is published with the approval of the Director.

Keywords

Central America
ELISA
Equine protozoal myeloencephalitis
Seroprevalence
Surface antigens

ASJC Scopus subject areas

Parasitology
Animal Science and Zoology
Veterinary (miscellaneous)
Insect Science
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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title = "Prevalence of antibodies to Sarcocystis neurona and Neospora hughesi in horses from Mexico",

abstract = "Equine protozoal myeloencephalitis (EPM) is a debilitating disease of horses caused by Sarcocystis neurona and Neospora hughesi. Sera from 495 horses in Durango State, Mexico were tested for anti-protozoal antibodies using enzyme-linked immunosorbent assays (ELISAs) based on major surface antigens of these two parasites. Antibodies to S. neurona were detected in 240 (48.5%) of the 495 horse sera tested with the rSnSAG2/4/3 trivalent ELISA. Multivariate analysis showed that exposure to S. neurona was associated with age, feeding grains and crops, and small herd size. Antibodies to N. hughesi were found in 15 (3.0%) of the 495 horse sera tested with the rNhSAG1 ELISA and confirmed by Western blot of N. hughesi tachyzoite antigen. This is the first report of S. neurona and N. hughesi exposure in horses in Mexico, and it affirms that EPM should be in the differential diagnosis for horses exhibiting signs of neurologic disease in this country.",

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author = "Yeargan, {Michelle R.} and Cosme Alvarado-Esquivel and Dubey, {Jitender P.} and Howe, {Daniel K.}",

note = "Funding Information: This research was funded partly by the Amerman Family Equine Research Endowment. The information reported in this paper (Manuscript #13-14-109) is part of a project of the Kentucky Agricultural Experiment Station and is published with the approval of the Director.",

year = "2013",

doi = "10.1051/parasite/2013029",

language = "English",

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AU - Yeargan, Michelle R.

AU - Alvarado-Esquivel, Cosme

AU - Dubey, Jitender P.

AU - Howe, Daniel K.

N1 - Funding Information: This research was funded partly by the Amerman Family Equine Research Endowment. The information reported in this paper (Manuscript #13-14-109) is part of a project of the Kentucky Agricultural Experiment Station and is published with the approval of the Director.

PY - 2013

Y1 - 2013

N2 - Equine protozoal myeloencephalitis (EPM) is a debilitating disease of horses caused by Sarcocystis neurona and Neospora hughesi. Sera from 495 horses in Durango State, Mexico were tested for anti-protozoal antibodies using enzyme-linked immunosorbent assays (ELISAs) based on major surface antigens of these two parasites. Antibodies to S. neurona were detected in 240 (48.5%) of the 495 horse sera tested with the rSnSAG2/4/3 trivalent ELISA. Multivariate analysis showed that exposure to S. neurona was associated with age, feeding grains and crops, and small herd size. Antibodies to N. hughesi were found in 15 (3.0%) of the 495 horse sera tested with the rNhSAG1 ELISA and confirmed by Western blot of N. hughesi tachyzoite antigen. This is the first report of S. neurona and N. hughesi exposure in horses in Mexico, and it affirms that EPM should be in the differential diagnosis for horses exhibiting signs of neurologic disease in this country.

AB - Equine protozoal myeloencephalitis (EPM) is a debilitating disease of horses caused by Sarcocystis neurona and Neospora hughesi. Sera from 495 horses in Durango State, Mexico were tested for anti-protozoal antibodies using enzyme-linked immunosorbent assays (ELISAs) based on major surface antigens of these two parasites. Antibodies to S. neurona were detected in 240 (48.5%) of the 495 horse sera tested with the rSnSAG2/4/3 trivalent ELISA. Multivariate analysis showed that exposure to S. neurona was associated with age, feeding grains and crops, and small herd size. Antibodies to N. hughesi were found in 15 (3.0%) of the 495 horse sera tested with the rNhSAG1 ELISA and confirmed by Western blot of N. hughesi tachyzoite antigen. This is the first report of S. neurona and N. hughesi exposure in horses in Mexico, and it affirms that EPM should be in the differential diagnosis for horses exhibiting signs of neurologic disease in this country.

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KW - Seroprevalence

KW - Surface antigens

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Prevalence of antibodies to Sarcocystis neurona and Neospora hughesi in horses from Mexico

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

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