Prevention and Detection of Fungicide Resistance Development in Rhizoctonia zeae from Soybean and Corn in Nebraska

Nikita Gambhir; Srikanth Kodati; Matthew Huff; Flavio Silva; Olutoyosi Ajayi-Oyetunde; Margaret Staton; Carl Bradley; Anthony O. Adesemoye; Sydney E. Everhart

doi:10.1094/PHP-11-20-0100-SYN

Prevention and Detection of Fungicide Resistance Development in Rhizoctonia zeae from Soybean and Corn in Nebraska

Nikita Gambhir, Srikanth Kodati, Matthew Huff, Flavio Silva, Olutoyosi Ajayi-Oyetunde, Margaret Staton, Carl Bradley, Anthony O. Adesemoye, Sydney E. Everhart

Plant Pathology

Research output: Contribution to journal › Article › peer-review

Abstract

Thegoalofthisresearch was to advance the foundational knowledge required to quantify and mitigate fungicide resistance in Rhizoctonia zeae, the seedling disease pathogen ofsoybean and corn. In vitro sensitivity to azoxystrobin, fludioxonil, sedaxane, and/or prothioconazole was determined for 91 R. zeae isolates obtained mostly from soybean and corn fields in Nebraska. Isolates were sensitive to flu-dioxonil, sedaxane, and prothioconazole (EC₅₀ <3 µg/ml) and had a positively skewed EC50 distribution. Isolates were not sensitive to azoxystrobin in vitro (EC₅₀ > 100 µg/ml) or in planta. Application of azoxystrobin did not significantly decrease disease severity or improve total dry weight of the soybean plants (P > 0.05). The risk of resistance developmentin R. zeae was estimated by characterizing its population structure. Eighty-one R. zeae isolates were genotyped using six microsatellite markers. Results showed that the population has a mixed mode of reproduction and is structured according to geographic region, suggesting limited dispersal. These population characteristics suggest that R. zeae has an intermediate risk of resistance development. Overall, this research establishedthecurrent status of fungicide sensitivity in R. zeae in Nebraska and estimated its risk of resistance development, which can inform fungicide resistance management for R. zeae.

Original language	English
Pages (from-to)	465-469
Number of pages	5
Journal	Plant Health Progress
Volume	22
Issue number	4
DOIs	https://doi.org/10.1094/PHP-11-20-0100-SYN
State	Published - 2021

Bibliographical note

Funding Information:
Funding: This work was partially completed utilizing the Holland Computing Center of the University of Nebraska, which receives support from the Nebraska Research Initiative. Funding for this project was provided by the Nebraska Soybean Board (16R-21-1/3 #1720) and the North Central Soybean Research Program (SIU Carbondale 16-13). Funding for communicating the results of this project at Plant Health 2020 was provided by the I. E. Melhus Symposium.

Publisher Copyright:
© 2021 The American Phytopathological Society

Keywords

Azoxystrobin
Fludioxonil
Fungicide resistance management
Fungicide sensitivity
Glycine max
Population structure
Prothioconazole
Risk of resistance development
Sedaxane
Zea mays

ASJC Scopus subject areas

Plant Science
Horticulture

Access to Document

10.1094/PHP-11-20-0100-SYN

Cite this

@article{b2d2b8df3a8645dcbb22f05920f53091,

title = "Prevention and Detection of Fungicide Resistance Development in Rhizoctonia zeae from Soybean and Corn in Nebraska",

abstract = "Thegoalofthisresearch was to advance the foundational knowledge required to quantify and mitigate fungicide resistance in Rhizoctonia zeae, the seedling disease pathogen ofsoybean and corn. In vitro sensitivity to azoxystrobin, fludioxonil, sedaxane, and/or prothioconazole was determined for 91 R. zeae isolates obtained mostly from soybean and corn fields in Nebraska. Isolates were sensitive to flu-dioxonil, sedaxane, and prothioconazole (EC50 <3 µg/ml) and had a positively skewed EC50 distribution. Isolates were not sensitive to azoxystrobin in vitro (EC50 > 100 µg/ml) or in planta. Application of azoxystrobin did not significantly decrease disease severity or improve total dry weight of the soybean plants (P > 0.05). The risk of resistance developmentin R. zeae was estimated by characterizing its population structure. Eighty-one R. zeae isolates were genotyped using six microsatellite markers. Results showed that the population has a mixed mode of reproduction and is structured according to geographic region, suggesting limited dispersal. These population characteristics suggest that R. zeae has an intermediate risk of resistance development. Overall, this research establishedthecurrent status of fungicide sensitivity in R. zeae in Nebraska and estimated its risk of resistance development, which can inform fungicide resistance management for R. zeae.",

keywords = "Azoxystrobin, Fludioxonil, Fungicide resistance management, Fungicide sensitivity, Glycine max, Population structure, Prothioconazole, Risk of resistance development, Sedaxane, Zea mays",

author = "Nikita Gambhir and Srikanth Kodati and Matthew Huff and Flavio Silva and Olutoyosi Ajayi-Oyetunde and Margaret Staton and Carl Bradley and Adesemoye, {Anthony O.} and Everhart, {Sydney E.}",

note = "Funding Information: Funding: This work was partially completed utilizing the Holland Computing Center of the University of Nebraska, which receives support from the Nebraska Research Initiative. Funding for this project was provided by the Nebraska Soybean Board (16R-21-1/3 #1720) and the North Central Soybean Research Program (SIU Carbondale 16-13). Funding for communicating the results of this project at Plant Health 2020 was provided by the I. E. Melhus Symposium. Publisher Copyright: {\textcopyright} 2021 The American Phytopathological Society",

year = "2021",

doi = "10.1094/PHP-11-20-0100-SYN",

language = "English",

volume = "22",

pages = "465--469",

number = "4",

}

TY - JOUR

T1 - Prevention and Detection of Fungicide Resistance Development in Rhizoctonia zeae from Soybean and Corn in Nebraska

AU - Gambhir, Nikita

AU - Kodati, Srikanth

AU - Huff, Matthew

AU - Silva, Flavio

AU - Ajayi-Oyetunde, Olutoyosi

AU - Staton, Margaret

AU - Bradley, Carl

AU - Adesemoye, Anthony O.

AU - Everhart, Sydney E.

N1 - Funding Information: Funding: This work was partially completed utilizing the Holland Computing Center of the University of Nebraska, which receives support from the Nebraska Research Initiative. Funding for this project was provided by the Nebraska Soybean Board (16R-21-1/3 #1720) and the North Central Soybean Research Program (SIU Carbondale 16-13). Funding for communicating the results of this project at Plant Health 2020 was provided by the I. E. Melhus Symposium. Publisher Copyright: © 2021 The American Phytopathological Society

PY - 2021

Y1 - 2021

N2 - Thegoalofthisresearch was to advance the foundational knowledge required to quantify and mitigate fungicide resistance in Rhizoctonia zeae, the seedling disease pathogen ofsoybean and corn. In vitro sensitivity to azoxystrobin, fludioxonil, sedaxane, and/or prothioconazole was determined for 91 R. zeae isolates obtained mostly from soybean and corn fields in Nebraska. Isolates were sensitive to flu-dioxonil, sedaxane, and prothioconazole (EC50 <3 µg/ml) and had a positively skewed EC50 distribution. Isolates were not sensitive to azoxystrobin in vitro (EC50 > 100 µg/ml) or in planta. Application of azoxystrobin did not significantly decrease disease severity or improve total dry weight of the soybean plants (P > 0.05). The risk of resistance developmentin R. zeae was estimated by characterizing its population structure. Eighty-one R. zeae isolates were genotyped using six microsatellite markers. Results showed that the population has a mixed mode of reproduction and is structured according to geographic region, suggesting limited dispersal. These population characteristics suggest that R. zeae has an intermediate risk of resistance development. Overall, this research establishedthecurrent status of fungicide sensitivity in R. zeae in Nebraska and estimated its risk of resistance development, which can inform fungicide resistance management for R. zeae.

AB - Thegoalofthisresearch was to advance the foundational knowledge required to quantify and mitigate fungicide resistance in Rhizoctonia zeae, the seedling disease pathogen ofsoybean and corn. In vitro sensitivity to azoxystrobin, fludioxonil, sedaxane, and/or prothioconazole was determined for 91 R. zeae isolates obtained mostly from soybean and corn fields in Nebraska. Isolates were sensitive to flu-dioxonil, sedaxane, and prothioconazole (EC50 <3 µg/ml) and had a positively skewed EC50 distribution. Isolates were not sensitive to azoxystrobin in vitro (EC50 > 100 µg/ml) or in planta. Application of azoxystrobin did not significantly decrease disease severity or improve total dry weight of the soybean plants (P > 0.05). The risk of resistance developmentin R. zeae was estimated by characterizing its population structure. Eighty-one R. zeae isolates were genotyped using six microsatellite markers. Results showed that the population has a mixed mode of reproduction and is structured according to geographic region, suggesting limited dispersal. These population characteristics suggest that R. zeae has an intermediate risk of resistance development. Overall, this research establishedthecurrent status of fungicide sensitivity in R. zeae in Nebraska and estimated its risk of resistance development, which can inform fungicide resistance management for R. zeae.

KW - Azoxystrobin

KW - Fludioxonil

KW - Fungicide resistance management

KW - Fungicide sensitivity

KW - Glycine max

KW - Population structure

KW - Prothioconazole

KW - Risk of resistance development

KW - Sedaxane

KW - Zea mays

UR - http://www.scopus.com/inward/record.url?scp=85123802690&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85123802690&partnerID=8YFLogxK

U2 - 10.1094/PHP-11-20-0100-SYN

DO - 10.1094/PHP-11-20-0100-SYN

M3 - Article

AN - SCOPUS:85123802690

VL - 22

SP - 465

EP - 469

IS - 4

ER -

Prevention and Detection of Fungicide Resistance Development in Rhizoctonia zeae from Soybean and Corn in Nebraska

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this