TY - JOUR
T1 - Prevention of TGFβ induction attenuates angII -stimulated vascular biglycan and atherosclerosis in Ldlr-/- mice1
AU - Tang, Tao
AU - Wilson, Patricia G.
AU - Thompson, Joel C.
AU - Nelson, Christina
AU - Yoder, Meghan H.
AU - Tannock, Lisa R.
PY - 2013/8
Y1 - 2013/8
N2 - Angiotensin II (angII) accelerates atherosclerosis, but the mechanisms are not fully understood. The aim of this study was to determine whether TGFβ is required for angIIinduced atherosclerosis. Ldlr -null mice fed a normal chow diet were infused with angII or saline for 28 days. A single injection of TGFβ neutralizing antibody 1D11 (2 mg/kg) prevented angII-induction of TGFβ 1 levels, and strikingly attenuated angII-induced accumulation of aortic biglycan content. To study atherosclerosis, mice were infused with angII or saline for 4 weeks, and then fed Western diet for a further 6 weeks. 1D11 had no effect on systolic blood pressure or plasma cholesterol; however, angII-infused mice that received 1D11 had reduced atherosclerotic lesion area by 30% ( P < 0.05). Immunohistochemical analyses demonstrated that angII induced both lipid retention and accumulation of biglycan and perlecan which colocalized with apoB. 1D11 strikingly reduced the effect of angII on biglycan but not perlecan. 1D11 decreased total collagen content ( P < 0.05) in the lesion area without changing plaque inflammation markers (CD68 and CD45). Thus, this study demonstrates that neutralization of TGFβ attenuated angII stimulation of biglycan accumulation and atherogenesis in mice, suggesting that TGFβ -mediated biglycan induction is one of the mechanisms underlying angII-promoted atherosclerosis.
AB - Angiotensin II (angII) accelerates atherosclerosis, but the mechanisms are not fully understood. The aim of this study was to determine whether TGFβ is required for angIIinduced atherosclerosis. Ldlr -null mice fed a normal chow diet were infused with angII or saline for 28 days. A single injection of TGFβ neutralizing antibody 1D11 (2 mg/kg) prevented angII-induction of TGFβ 1 levels, and strikingly attenuated angII-induced accumulation of aortic biglycan content. To study atherosclerosis, mice were infused with angII or saline for 4 weeks, and then fed Western diet for a further 6 weeks. 1D11 had no effect on systolic blood pressure or plasma cholesterol; however, angII-infused mice that received 1D11 had reduced atherosclerotic lesion area by 30% ( P < 0.05). Immunohistochemical analyses demonstrated that angII induced both lipid retention and accumulation of biglycan and perlecan which colocalized with apoB. 1D11 strikingly reduced the effect of angII on biglycan but not perlecan. 1D11 decreased total collagen content ( P < 0.05) in the lesion area without changing plaque inflammation markers (CD68 and CD45). Thus, this study demonstrates that neutralization of TGFβ attenuated angII stimulation of biglycan accumulation and atherogenesis in mice, suggesting that TGFβ -mediated biglycan induction is one of the mechanisms underlying angII-promoted atherosclerosis.
KW - Angiotensin II
KW - Atherosclerosis initiation
KW - Biglycan-apoB colocalization
KW - Low density lipoprotein retention
KW - Proteoglycans
KW - Renin-angiotensin system
KW - TGFβ neutralizing antibody
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U2 - 10.1194/jlr.P040139
DO - 10.1194/jlr.P040139
M3 - Article
C2 - 23749984
AN - SCOPUS:84881226028
SN - 0022-2275
VL - 54
SP - 2255
EP - 2264
JO - Journal of Lipid Research
JF - Journal of Lipid Research
IS - 8
ER -