5-epi-Aristolochene dihydroxylase (EAH) catalyzes unique stereo- and regiospecific hydroxylations of a bicyclic sesquiterpene hydrocarbon to generate capsidiol. To define functional and mechanistic features of the EAH enzyme, the utility of a coupled assay using readily available sesquiterpene synthases and microsomes from yeast overexpressing the EAH enzyme was determined. Capsidiol and deoxycapsidiol biosyntheses were readily measured in coupled assays consisting of 5-epi-aristolochene synthase and EAH as determined by the incorporation of radiolabeled farnesyl diphosphate into thin-layer chromatography-isolated products and verified by gas chromatography-mass spectrometry analysis. The assays were dependent on the amounts of synthase and hydroxylase protein added, the incubation times, and the presence of nicotinamide adenine dinucleotide phosphate. The utility of this coupled assay was extended by examining the relative efficiency of the EAH enzyme to catalyze hydroxylations of different sesquiterpene skeletons generated by other terpene synthases.
|Number of pages||10|
|Journal||Archives of Biochemistry and Biophysics|
|State||Published - Jan 15 2003|
Bibliographical noteFunding Information:
The Kentucky Agricultural Experiment Station and a grant from the NSF supported this work.
- 5-epi-Aristolochene dihydroxylase hydroxylase
- 5-epi-Aristolochene synthase
- Germacrene A
- Germacrene diol
- Premnaspirodiene synthase
ASJC Scopus subject areas
- Molecular Biology