Production of soluble suppressor factors by herpes simplex virus-stimulated splenocytes from herpes simplex virus-immune mice

D. W. Horohov, R. N. Moore, B. T. Rouse

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Indirect evidence indicates that herpes simplex virus (HSV)-specific cytotoxic T-lymphocyte induction is regulated by suppressor cells. To search for such suppressor effects, supernatant fluids from splenocyte cultures from normal and HSV-immune mice cultured either with or without viral stimulation were tested for their ability to inhibit HSV-specific cytotoxic-T-lymphocyte induction. Only the supernatant fluid from the HSV-stimulated, HSV-immune cultures contained a suppressor actvity (HSV-SF). HSV-SF was produced by nylon-wool-purified Thy 1+ cells. HSV-SF was detectable after 3 days of culture and would only suppress cytotoxic-T-lymphocyte induction if HSV-SF was added within 24 h of initiation of the test cultures. HSV-SF was neither dialyzable nor heat stable. Molecular sieve chromatography of HSV-SF yielded multiple peaks of suppressor activity. Although most of these peaks exhibited nonspecific suppressor activity, the suppression mediated by the 90,000 to 150,000-molecular-weight fractions was antigen specific and genetically restricted. These results provide direct evidence for the regulation of HSV-cytotoxic-T-lymphocyte induction by a noval suppressor factor.

Original languageEnglish
Pages (from-to)798-803
Number of pages6
JournalJournal of Virology
Volume54
Issue number3
DOIs
StatePublished - 1985

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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