Abstract
Indirect evidence indicates that herpes simplex virus (HSV)-specific cytotoxic T-lymphocyte induction is regulated by suppressor cells. To search for such suppressor effects, supernatant fluids from splenocyte cultures from normal and HSV-immune mice cultured either with or without viral stimulation were tested for their ability to inhibit HSV-specific cytotoxic-T-lymphocyte induction. Only the supernatant fluid from the HSV-stimulated, HSV-immune cultures contained a suppressor actvity (HSV-SF). HSV-SF was produced by nylon-wool-purified Thy 1+ cells. HSV-SF was detectable after 3 days of culture and would only suppress cytotoxic-T-lymphocyte induction if HSV-SF was added within 24 h of initiation of the test cultures. HSV-SF was neither dialyzable nor heat stable. Molecular sieve chromatography of HSV-SF yielded multiple peaks of suppressor activity. Although most of these peaks exhibited nonspecific suppressor activity, the suppression mediated by the 90,000 to 150,000-molecular-weight fractions was antigen specific and genetically restricted. These results provide direct evidence for the regulation of HSV-cytotoxic-T-lymphocyte induction by a noval suppressor factor.
Original language | English |
---|---|
Pages (from-to) | 798-803 |
Number of pages | 6 |
Journal | Journal of Virology |
Volume | 54 |
Issue number | 3 |
DOIs | |
State | Published - 1985 |
ASJC Scopus subject areas
- Microbiology
- Immunology
- Insect Science
- Virology