Progeny analysis of tobacco that express a mammalian Δ9 desaturase

W. S. Grayburn; D. F. Hildebrand

doi:10.1007/BF02541089

Progeny analysis of tobacco that express a mammalian Δ9 desaturase

W. S. Grayburn, D. F. Hildebrand

Plant and Soil Sciences

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9 Scopus citations

Abstract

Various tissues of Nicotiana tabacum plants transformed with a rat stearyl-CoA desaturase gene were analyzed for lipid composition. Gas chromatographic analysis showed a decrease in saturated 16 and 18 carbon fatty acids in leaves and roots of several progeny. Polymerase chain reaction analysis of total DNA from leaves indicated the presence of the desaturase gene in kanamycin-resistant progeny with altered fatty acid composition. Western immunoblot analysis confirmed the presence of introduced desaturase protein. Data on the inheritance of kanamycin resistance is presented.

Original language	English
Pages (from-to)	317-321
Number of pages	5
Journal	Journal of the American Oil Chemists' Society
Volume	72
Issue number	3
DOIs	https://doi.org/10.1007/BF02541089
State	Published - Mar 1995

Keywords

Fatty acids
lipids
palmitic acid
vegetable oil quality

ASJC Scopus subject areas

Chemical Engineering (all)
Organic Chemistry

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10.1007/BF02541089

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title = "Progeny analysis of tobacco that express a mammalian Δ9 desaturase",

abstract = "Various tissues of Nicotiana tabacum plants transformed with a rat stearyl-CoA desaturase gene were analyzed for lipid composition. Gas chromatographic analysis showed a decrease in saturated 16 and 18 carbon fatty acids in leaves and roots of several progeny. Polymerase chain reaction analysis of total DNA from leaves indicated the presence of the desaturase gene in kanamycin-resistant progeny with altered fatty acid composition. Western immunoblot analysis confirmed the presence of introduced desaturase protein. Data on the inheritance of kanamycin resistance is presented.",

keywords = "Fatty acids, lipids, palmitic acid, vegetable oil quality",

author = "Grayburn, {W. S.} and Hildebrand, {D. F.}",

year = "1995",

month = mar,

doi = "10.1007/BF02541089",

language = "English",

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pages = "317--321",

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T1 - Progeny analysis of tobacco that express a mammalian Δ9 desaturase

AU - Grayburn, W. S.

AU - Hildebrand, D. F.

PY - 1995/3

Y1 - 1995/3

N2 - Various tissues of Nicotiana tabacum plants transformed with a rat stearyl-CoA desaturase gene were analyzed for lipid composition. Gas chromatographic analysis showed a decrease in saturated 16 and 18 carbon fatty acids in leaves and roots of several progeny. Polymerase chain reaction analysis of total DNA from leaves indicated the presence of the desaturase gene in kanamycin-resistant progeny with altered fatty acid composition. Western immunoblot analysis confirmed the presence of introduced desaturase protein. Data on the inheritance of kanamycin resistance is presented.

AB - Various tissues of Nicotiana tabacum plants transformed with a rat stearyl-CoA desaturase gene were analyzed for lipid composition. Gas chromatographic analysis showed a decrease in saturated 16 and 18 carbon fatty acids in leaves and roots of several progeny. Polymerase chain reaction analysis of total DNA from leaves indicated the presence of the desaturase gene in kanamycin-resistant progeny with altered fatty acid composition. Western immunoblot analysis confirmed the presence of introduced desaturase protein. Data on the inheritance of kanamycin resistance is presented.

KW - Fatty acids

KW - lipids

KW - palmitic acid

KW - vegetable oil quality

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JF - Journal of the American Oil Chemists' Society

IS - 3

ER -

Progeny analysis of tobacco that express a mammalian Δ9 desaturase

Abstract

Keywords

ASJC Scopus subject areas

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