Progeny analysis of tobacco that express a mammalian Δ9 desaturase

W. S. Grayburn, D. F. Hildebrand

Research output: Contribution to journalArticlepeer-review

9 Scopus citations


Various tissues of Nicotiana tabacum plants transformed with a rat stearyl-CoA desaturase gene were analyzed for lipid composition. Gas chromatographic analysis showed a decrease in saturated 16 and 18 carbon fatty acids in leaves and roots of several progeny. Polymerase chain reaction analysis of total DNA from leaves indicated the presence of the desaturase gene in kanamycin-resistant progeny with altered fatty acid composition. Western immunoblot analysis confirmed the presence of introduced desaturase protein. Data on the inheritance of kanamycin resistance is presented.

Original languageEnglish
Pages (from-to)317-321
Number of pages5
JournalJournal of the American Oil Chemists' Society
Issue number3
StatePublished - Mar 1995


  • Fatty acids
  • lipids
  • palmitic acid
  • vegetable oil quality

ASJC Scopus subject areas

  • Chemical Engineering (all)
  • Organic Chemistry


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