Programming for the DNA analysis of FCM data on an IBM microcomputer

Edward J. Pavlik, Robert C. Flanigan, Jonathan Bartmas, John R. Van Nagell, Elvis S. Donaldson, Michael B. Hanson, Holly Gallion, Daniel E. Kenady

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


The analysis of data generated on a flow cytometer (FCM) is often performed on a computer obtained especially for dedicated use with the flow cytometer. This computer component can be expensive and also presents the FCM user with the added burden of mastering specialized programming language or of accepting the secret analytical processes of protected proprietary program routines. We believe that the evolution of more accurate and efficient FCM analyses that have the power to consider complex signal distributions can be assisted by the availability of analysis programs written in languages common to many users. DNA analysis routines written for a relatively inexpensive micro‐computer (IBM PC/XT) in Basic and Pascal are described here. The routines can automatically process multiple FCM data files and can provide high‐resolution graphic hardcopy. A foreground/background utilization is also described that allows the computer to be available for other uses in the laboratory.

Original languageEnglish
Pages (from-to)93-97
Number of pages5
Issue number1
StatePublished - Jan 1986


  • Basic
  • Fried method
  • Pascal
  • foreground/background operation
  • high‐resolution graphics

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Biophysics
  • Hematology
  • Endocrinology
  • Cell Biology


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