Injectable hydrogels can generate and support pro-repair environments in injured tissue. Here we used a slow-releasing drug carrying in situ-forming hydrogel to promote post-stroke recovery in a rat model. Release kinetics were measured in vitro and in vivo with MRI, using gadolinium-labeled albumin (Galbumin), which demonstrated prolonged release over multiple weeks. Subsequently, this hydrogel was used for long-term delivery of vascular endothelial growth factor (VEGF) and angiopoietin-1 (Ang1) (Gel VEGF + Ang1, n = 14), in a photothrombotically induced cortical stroke lesion in rats. Control stroke animals were intralesionally injected with saline (Saline, n = 10), non-loaded gel (Gel, n = 10), or a single bolus of VEGF + Ang1 in saline (Saline VEGF + Ang1, n = 10). MRI was executed to guide hydrogel injection. Functional recovery was assessed with sensorimotor function tests, while tissue status and vascularization were monitored by serial in vivo MRI. Significant recovery from sensorimotor deficits from day 28 onwards was only measured in the Gel VEGF + Ang1 group. This was accompanied by significantly increased vascularization in the perilesional cortex. Histology confirmed (re)vascularization and neuronal sparing in perilesional areas. In conclusion, intralesional injection of in situ-forming hydrogel loaded with pro-angiogenic factors can support prolonged brain tissue regeneration and promote functional recovery in the chronic phase post-stroke.
|Number of pages||16|
|Journal||Journal of Cerebral Blood Flow and Metabolism|
|State||Published - Jun 2022|
Bibliographical noteFunding Information:
The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This work was supported by the Netherlands Organization for Scientific Research (VICI 016.130.662 to R.M.D.), Texas Institute for Brain Injury and Repair (TIBIR to A.M.S.), and The Beatrice Menne Haggerty Center (UTSW to A.M.S.). Acknowledgments
The authors thank Erik Plautz, Ph.D. and Laura Doss, M.S. for input and feedback on the evaluation of the behavioral data. The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This work was supported by the Netherlands Organization for Scientific Research (VICI 016.130.662 to R.M.D.), Texas Institute for Brain Injury and Repair (TIBIR to A.M.S.), and The Beatrice Menne Haggerty Center (UTSW to A.M.S.).
© The Author(s) 2022.
- functional recovery
- hydrogel drug-delivery
ASJC Scopus subject areas
- Clinical Neurology
- Cardiology and Cardiovascular Medicine