Promoter analysis of ventricular myosin heavy chain (vmhc) in zebrafish embryos

Daqing Jin, Terri T. Ni, Jia Hou, Eric Rellinger, Tao P. Zhong

Research output: Contribution to journalArticlepeer-review

31 Scopus citations

Abstract

In zebrafish, ventricular myosin heavy chain (vmhc) gene is initially expressed at the anterior lateral mesoderm and thereafter its expression is restricted to the cardiac ventricle. The transcriptional control mechanisms in regulating chamber-specific expression of myosin heavy chains are not well defined. We isolated and analyzed zebrafish vmhc upstream region to examine the spatial and temporal regulation of vmhc using transgenic and transient expression techniques. Promoter deletion analyses defined a basal promoter region sufficient to drive vmhc expression in the ventricle and an upstream fragment necessary for repressing ectopic vmhc expression in the atrium. The transcriptional mechanism that prevents vmhc expression in the atrium is mediated through Nkx2.5 binding elements (NKE). We have further discovered that paired-related homeobox transcriptional factor 2 (Prx2/S8)-like binding elements are required for promoting vmhc expression, and Prrx1b, a Prx-related homeobox protein, participates in the regulation of vmhc expression with other transcriptional factors.

Original languageEnglish
Pages (from-to)1760-1767
Number of pages8
JournalDevelopmental Dynamics
Volume238
Issue number7
DOIs
StatePublished - Jul 2009

Keywords

  • Cardiac chamber
  • Promoter analysis
  • Transgene
  • Vmhc
  • Zebrafish

ASJC Scopus subject areas

  • Developmental Biology

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