Dulbecco's modified Eagle's medium promotes aggregation and fibrillization of the synthetic amyloid β1-40 and β1-42 peptides more than RPMI and OPTI media. Fibrillization in all of these media is faster than in phosphate-buffered saline and Tris buffer. Normal and heat- inactivated fetal bovine and human serum abolish amyloid fibril formation in buffers and cell culture media. Fibrillar amyloid formed during 2-day-long incubation in cell culture media and buffers is defibrillized by 1-day-long treatment with human and bovine serum. This study indicates that amyloid β fibrillogenesis in cell culture should be studied in serum-free media or in media with a low concentration of serum.
|Number of pages
|Published - Jun 28 1996
Bibliographical noteFunding Information:
The authors wish to thank Ms. Maureen Stoddard Marlow for copy-editingth e manuscriptS. upportedin part by funds from the New York StateO ffice of Mental Retardationa nd DevelopmentaDli sabilitiesa nd a grant from the NationalI nstituteso f Health, National Institute of Aging No. PO1-AGO-4220.
- Amyloid β
- Cell culture media
ASJC Scopus subject areas
- Neuroscience (all)