Promotion of synthetic amyloid β-peptide fibrillization by cell culture media and cessation of fibrillization by serum

Jerzy Wegiel, Abha Chauhan, Henryk M. Wisniewski, Joseph Nowakowski, Kuo Chiang Wang, Harry Le Vine

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

Dulbecco's modified Eagle's medium promotes aggregation and fibrillization of the synthetic amyloid β1-40 and β1-42 peptides more than RPMI and OPTI media. Fibrillization in all of these media is faster than in phosphate-buffered saline and Tris buffer. Normal and heat- inactivated fetal bovine and human serum abolish amyloid fibril formation in buffers and cell culture media. Fibrillar amyloid formed during 2-day-long incubation in cell culture media and buffers is defibrillized by 1-day-long treatment with human and bovine serum. This study indicates that amyloid β fibrillogenesis in cell culture should be studied in serum-free media or in media with a low concentration of serum.

Original languageEnglish
Pages (from-to)151-154
Number of pages4
JournalNeuroscience Letters
Volume211
Issue number3
DOIs
StatePublished - Jun 28 1996

Bibliographical note

Funding Information:
The authors wish to thank Ms. Maureen Stoddard Marlow for copy-editingth e manuscriptS. upportedin part by funds from the New York StateO ffice of Mental Retardationa nd DevelopmentaDli sabilitiesa nd a grant from the NationalI nstituteso f Health, National Institute of Aging No. PO1-AGO-4220.

Keywords

  • Amyloid β
  • Cell culture media
  • Fibrillization
  • Serum

ASJC Scopus subject areas

  • Neuroscience (all)

Fingerprint

Dive into the research topics of 'Promotion of synthetic amyloid β-peptide fibrillization by cell culture media and cessation of fibrillization by serum'. Together they form a unique fingerprint.

Cite this