Promotion of synthetic amyloid β-peptide fibrillization by cell culture media and cessation of fibrillization by serum

Jerzy Wegiel; Abha Chauhan; Henryk M. Wisniewski; Joseph Nowakowski; Kuo Chiang Wang; Harry Le Vine

doi:10.1016/0304-3940(96)12739-7

Promotion of synthetic amyloid β-peptide fibrillization by cell culture media and cessation of fibrillization by serum

Jerzy Wegiel, Abha Chauhan, Henryk M. Wisniewski, Joseph Nowakowski, Kuo Chiang Wang, Harry Le Vine

Molecular and Cellular Biochemistry

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

Dulbecco's modified Eagle's medium promotes aggregation and fibrillization of the synthetic amyloid β_1-40 and β_1-42 peptides more than RPMI and OPTI media. Fibrillization in all of these media is faster than in phosphate-buffered saline and Tris buffer. Normal and heat- inactivated fetal bovine and human serum abolish amyloid fibril formation in buffers and cell culture media. Fibrillar amyloid formed during 2-day-long incubation in cell culture media and buffers is defibrillized by 1-day-long treatment with human and bovine serum. This study indicates that amyloid β fibrillogenesis in cell culture should be studied in serum-free media or in media with a low concentration of serum.

Original language	English
Pages (from-to)	151-154
Number of pages	4
Journal	Neuroscience Letters
Volume	211
Issue number	3
DOIs	https://doi.org/10.1016/0304-3940(96)12739-7
State	Published - Jun 28 1996

Bibliographical note

Funding Information:
The authors wish to thank Ms. Maureen Stoddard Marlow for copy-editingth e manuscriptS. upportedin part by funds from the New York StateO ffice of Mental Retardationa nd DevelopmentaDli sabilitiesa nd a grant from the NationalI nstituteso f Health, National Institute of Aging No. PO1-AGO-4220.

Funding

The authors wish to thank Ms. Maureen Stoddard Marlow for copy-editingth e manuscriptS. upportedin part by funds from the New York StateO ffice of Mental Retardationa nd DevelopmentaDli sabilitiesa nd a grant from the NationalI nstituteso f Health, National Institute of Aging No. PO1-AGO-4220.

Funders	Funder number
NationalI nstituteso f Health
New York StateO ffice of Mental Retardationa
National Institute on Aging	P01AG004220

Keywords

Amyloid β
Cell culture media
Fibrillization
Serum

ASJC Scopus subject areas

General Neuroscience

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10.1016/0304-3940(96)12739-7

Cite this

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title = "Promotion of synthetic amyloid β-peptide fibrillization by cell culture media and cessation of fibrillization by serum",

abstract = "Dulbecco's modified Eagle's medium promotes aggregation and fibrillization of the synthetic amyloid β1-40 and β1-42 peptides more than RPMI and OPTI media. Fibrillization in all of these media is faster than in phosphate-buffered saline and Tris buffer. Normal and heat- inactivated fetal bovine and human serum abolish amyloid fibril formation in buffers and cell culture media. Fibrillar amyloid formed during 2-day-long incubation in cell culture media and buffers is defibrillized by 1-day-long treatment with human and bovine serum. This study indicates that amyloid β fibrillogenesis in cell culture should be studied in serum-free media or in media with a low concentration of serum.",

keywords = "Amyloid β, Cell culture media, Fibrillization, Serum",

author = "Jerzy Wegiel and Abha Chauhan and Wisniewski, {Henryk M.} and Joseph Nowakowski and Wang, {Kuo Chiang} and {Le Vine}, Harry",

note = "Funding Information: The authors wish to thank Ms. Maureen Stoddard Marlow for copy-editingth e manuscriptS. upportedin part by funds from the New York StateO ffice of Mental Retardationa nd DevelopmentaDli sabilitiesa nd a grant from the NationalI nstituteso f Health, National Institute of Aging No. PO1-AGO-4220.",

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AU - Wegiel, Jerzy

AU - Chauhan, Abha

AU - Wisniewski, Henryk M.

AU - Nowakowski, Joseph

AU - Wang, Kuo Chiang

AU - Le Vine, Harry

N1 - Funding Information: The authors wish to thank Ms. Maureen Stoddard Marlow for copy-editingth e manuscriptS. upportedin part by funds from the New York StateO ffice of Mental Retardationa nd DevelopmentaDli sabilitiesa nd a grant from the NationalI nstituteso f Health, National Institute of Aging No. PO1-AGO-4220.

PY - 1996/6/28

Y1 - 1996/6/28

N2 - Dulbecco's modified Eagle's medium promotes aggregation and fibrillization of the synthetic amyloid β1-40 and β1-42 peptides more than RPMI and OPTI media. Fibrillization in all of these media is faster than in phosphate-buffered saline and Tris buffer. Normal and heat- inactivated fetal bovine and human serum abolish amyloid fibril formation in buffers and cell culture media. Fibrillar amyloid formed during 2-day-long incubation in cell culture media and buffers is defibrillized by 1-day-long treatment with human and bovine serum. This study indicates that amyloid β fibrillogenesis in cell culture should be studied in serum-free media or in media with a low concentration of serum.

AB - Dulbecco's modified Eagle's medium promotes aggregation and fibrillization of the synthetic amyloid β1-40 and β1-42 peptides more than RPMI and OPTI media. Fibrillization in all of these media is faster than in phosphate-buffered saline and Tris buffer. Normal and heat- inactivated fetal bovine and human serum abolish amyloid fibril formation in buffers and cell culture media. Fibrillar amyloid formed during 2-day-long incubation in cell culture media and buffers is defibrillized by 1-day-long treatment with human and bovine serum. This study indicates that amyloid β fibrillogenesis in cell culture should be studied in serum-free media or in media with a low concentration of serum.

KW - Amyloid β

KW - Cell culture media

KW - Fibrillization

KW - Serum

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JO - Neuroscience Letters

JF - Neuroscience Letters

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Promotion of synthetic amyloid β-peptide fibrillization by cell culture media and cessation of fibrillization by serum

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

Access to Document

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