Properties of anticancer agents relevant to in vitro determinations of human tumor cell sensitivity

Edward J. Pavlik, Daniel E. Kenady, John R. van Nagell, Kathryn Keaton, Michael B. Hanson, Elvis S. Donaldson, Ward O. Griffen, Robert C. Flanigan

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

The physical properties of 59 anticancer agents have been examined with respect to solubility in tissue culture media, binding to ultrafiltration materials, and molecular absorbance and fluorescence behavior. Methods for dissolving these agents, which are compatible with in vitro sensitivity testing of human tumor cells to anticancer agents, are reported in this paper. The potential for anticancer agent binding to cellulose nitrate/cellulose acetate and teflon membrane ultrafilters was documented, and quantitation of these anticancer agents based upon absorbance and fluorescence spectroscopy was performed. Post-filtration quantitation of anticancer agents was found to be a reliable method for determining the actual drug concentrations available in tumor cell sensitivity testing in vitro. The properties documented herein are pharmacologically relevant parameters related to in vitro determinations of human tumor cell sensitivity to anticancer agents.

Original languageEnglish
Pages (from-to)8-15
Number of pages8
JournalCancer Chemotherapy and Pharmacology
Volume11
Issue number1
DOIs
StatePublished - Aug 1983

ASJC Scopus subject areas

  • Oncology
  • Toxicology
  • Pharmacology
  • Cancer Research
  • Pharmacology (medical)

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