Properties of lanK-based regulatory circuit involved in landomycin biosynthesis in Streptomyces cyanogenus S136.

B. Ostash, I. Ostash, L. Zhu, M. K. Kharel, A. Luzhetskiǐ, A. Bechthold, S. Walker, J. Rohr, V. Fedorenko

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

LanK is TetR-like regulatory protein recently shown to regulate the export and glycosylation of landomycins in Streptomyces cyanogenus S136. Here, several properties of the lanK-mediated regulation were deciphered. LanK seems to function as oligomer as evident from experiments in vitro. In vivo, it is able to recognize various landomycins with altered aglycon structure and the minimal concentration of landomycin A sensed by LanK lies in low nanomolar range. Coexpression studies showed that the positive regulatory gene lanI upregulates lanK-dependent lan genes once the negative LanK-regulation is cancelled. Gene lanK can be useful for the construction of biosensor strains for sensitive and specific identification of producers of landomycin-like molecules with long glycosidic chains.

Original languageEnglish
Pages (from-to)604-609
Number of pages6
JournalGenetika
Volume46
Issue number5
StatePublished - May 2010

ASJC Scopus subject areas

  • Medicine (all)

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