Abstract
LanK is TetR-like regulatory protein recently shown to regulate the export and glycosylation of landomycins in Streptomyces cyanogenus S136. Here, several properties of the lanK-mediatcd regulation were deciphered. LanK seems to function as oligomer as evident from experiments in vitro. In vivo, it is able to recognize various landomycins with altered aglycon structure and the minimal concentration of landomycin A sensed by LanK lies in low nanomolar range. Coexpression studies showed that the positive regulatory gene lanI upregulates lanK-dependent lan genes once the negative LanK-regulation is cancelled. Gene lanK can be useful for the construction of biosensor strains for sensitive and specific identification of producers of landomycin-like molecules with long glycosidic chains.
Original language | English |
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Pages (from-to) | 530-535 |
Number of pages | 6 |
Journal | Russian Journal of Genetics |
Volume | 46 |
Issue number | 5 |
DOIs | |
State | Published - May 2010 |
Bibliographical note
Funding Information:strain. We are grateful to Prof. G. Padilla for the cos momycin D sample. This work was supported by grants Bg 01F and BG 201P from Ministry of Education and Science of Ukraine (to V.F.) and NIH grants AI50855 and CA102102 (to S.W. and J.R., respectively).
ASJC Scopus subject areas
- Genetics