protection by vitamin E against endothelial cell injury by linoleic acid hydroperoxides

Bernhard Hennig, Cynthia Enoch, Ching K. Chow

Research output: Contribution to journalArticlepeer-review

37 Scopus citations

Abstract

Exposure to 90 μM albumin-bound linoleic acid (18:2) for 24 hours significantly increased the rate of albumin transfer across cultured endothelial monolayers. Linoleic acid hydroperoxides (18:2-OOH) further accelerated the rate of albumin transfer over that of 18:2. Cells exposed to 18:2-OOH but not 18:2 caused a marked release of lactate dehydrogenase (LDH) into the media. Prior cellular incubation with media containing 25 μM vitamin E for 24 hours protected endothelial cells from injury by 18:2 and 18:2-OOH, as evidenced by a decreased rate of albumin transfer across the endothelium as well as reduced release of LDH into the media. These results suggest that vitamin E may provide protection against endothelial cell injury resulting from expusure to fatty acid hydroperoxides.

Original languageEnglish
Pages (from-to)1253-1259
Number of pages7
JournalNutrition Research
Volume7
Issue number12
DOIs
StatePublished - Dec 1987

Bibliographical note

Funding Information:
This research was supported in part by grant HL34423 from the National Heart, Lung and Blood Institute, National Institutes of Health; a grant from the American Heart Association, Kentucky Affiliate; and the University of Kentucky Agricultural Experiment Station.

Keywords

  • endothelial cell injury
  • lipid peroxidation
  • protection
  • vitamin E

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology
  • Nutrition and Dietetics

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