Abstract
Exposure to 90 μM albumin-bound linoleic acid (18:2) for 24 hours significantly increased the rate of albumin transfer across cultured endothelial monolayers. Linoleic acid hydroperoxides (18:2-OOH) further accelerated the rate of albumin transfer over that of 18:2. Cells exposed to 18:2-OOH but not 18:2 caused a marked release of lactate dehydrogenase (LDH) into the media. Prior cellular incubation with media containing 25 μM vitamin E for 24 hours protected endothelial cells from injury by 18:2 and 18:2-OOH, as evidenced by a decreased rate of albumin transfer across the endothelium as well as reduced release of LDH into the media. These results suggest that vitamin E may provide protection against endothelial cell injury resulting from expusure to fatty acid hydroperoxides.
| Original language | English |
|---|---|
| Pages (from-to) | 1253-1259 |
| Number of pages | 7 |
| Journal | Nutrition Research |
| Volume | 7 |
| Issue number | 12 |
| DOIs | |
| State | Published - Dec 1987 |
Bibliographical note
Funding Information:This research was supported in part by grant HL34423 from the National Heart, Lung and Blood Institute, National Institutes of Health; a grant from the American Heart Association, Kentucky Affiliate; and the University of Kentucky Agricultural Experiment Station.
Funding
This research was supported in part by grant HL34423 from the National Heart, Lung and Blood Institute, National Institutes of Health; a grant from the American Heart Association, Kentucky Affiliate; and the University of Kentucky Agricultural Experiment Station.
| Funders |
|---|
| Kentucky Affiliate |
| National Institutes of Health (NIH) |
| National Heart, Lung, and Blood Institute (NHLBI) |
| American Heart Association |
| Kentucky Agricultural Experiment Station |
Keywords
- endothelial cell injury
- lipid peroxidation
- protection
- vitamin E
ASJC Scopus subject areas
- Endocrinology, Diabetes and Metabolism
- Endocrinology
- Nutrition and Dietetics
