Protection of chickens from lethal influenza virus infection by influenza A/chicken/Pennsylvania/1/83 virus: Characterization of the protective effect

Thomas M. Chambers; Robert G. Webster

doi:10.1016/0042-6822(91)90160-D

Protection of chickens from lethal influenza virus infection by influenza A/chicken/Pennsylvania/1/83 virus: Characterization of the protective effect

Thomas M. Chambers, Robert G. Webster

Veterinary Science

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

The influenza A/chicken/Pennsylvania/1/83 (H5N2) virus is the first known example of an influenza virus isolated from a natural infection which contained primarily defective interfering particles (T. M. Chambers and R. G. Webster, J. Virol. 61, 1517-1523, 1987). In chickens, coinoculation of this virus together with the closely related but highly virulent influenza A/chicken/Pennsylvania/1370/83 virus results in reduced mortality compared to virulent virus infection alone(Bean et al., J. Virol. 54, 151-160, 1985). The biological basis of this protective effect has not been established. Protective activity required ≥100-fold excess input of protecting virus over virulent virus, functioned effectively during the first generations of virulent virus multiplication, and also functioned against an antigenically heterologous (H7N7) virulent influenza virus. Protection was correlated with the complete inhibition of virulent virus spread to the brain of infected chickens. Plaque-purified chicken/Pennsylvania/1/83 virus depleted of defective interfering particles, and β-propiolactone-inactivated virus, had no protective effect. These characteristics are consistent with the hypothesis that protection was the result of defective interfering particle-mediated interference with virulent virus multiplication within the respiratory tract of the chicken.

Original language	English
Pages (from-to)	427-432
Number of pages	6
Journal	Virology
Volume	183
Issue number	1
DOIs	https://doi.org/10.1016/0042-6822(91)90160-D
State	Published - Jul 1991

Bibliographical note

Funding Information:
This work was supported by U.S. Public Health Service Grant No. Al 29680 from the National Institute of Allergy and Infectious Diseases, by Cancer Center Support (CORE) Grant CA-21 765, and by American Lebanese Syrian Associated Charities. We thank Gary R. Burleson (U.S. Environmental Protection Agency) for help and advice regarding nonspecific immunity, and John Freeman and Tim Thomas for technical assistance.

Funding

This work was supported by U.S. Public Health Service Grant No. Al 29680 from the National Institute of Allergy and Infectious Diseases, by Cancer Center Support (CORE) Grant CA-21 765, and by American Lebanese Syrian Associated Charities. We thank Gary R. Burleson (U.S. Environmental Protection Agency) for help and advice regarding nonspecific immunity, and John Freeman and Tim Thomas for technical assistance.

Funders	Funder number
Cancer Center Support	CA-21 765
National Institute of Allergy and Infectious Diseases	R01AI029680
U.S. Public Health Service	29680
American Lebanese Syrian Associated Charities

ASJC Scopus subject areas

Virology

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10.1016/0042-6822(91)90160-D

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title = "Protection of chickens from lethal influenza virus infection by influenza A/chicken/Pennsylvania/1/83 virus: Characterization of the protective effect",

abstract = "The influenza A/chicken/Pennsylvania/1/83 (H5N2) virus is the first known example of an influenza virus isolated from a natural infection which contained primarily defective interfering particles (T. M. Chambers and R. G. Webster, J. Virol. 61, 1517-1523, 1987). In chickens, coinoculation of this virus together with the closely related but highly virulent influenza A/chicken/Pennsylvania/1370/83 virus results in reduced mortality compared to virulent virus infection alone(Bean et al., J. Virol. 54, 151-160, 1985). The biological basis of this protective effect has not been established. Protective activity required ≥100-fold excess input of protecting virus over virulent virus, functioned effectively during the first generations of virulent virus multiplication, and also functioned against an antigenically heterologous (H7N7) virulent influenza virus. Protection was correlated with the complete inhibition of virulent virus spread to the brain of infected chickens. Plaque-purified chicken/Pennsylvania/1/83 virus depleted of defective interfering particles, and β-propiolactone-inactivated virus, had no protective effect. These characteristics are consistent with the hypothesis that protection was the result of defective interfering particle-mediated interference with virulent virus multiplication within the respiratory tract of the chicken.",

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note = "Funding Information: This work was supported by U.S. Public Health Service Grant No. Al 29680 from the National Institute of Allergy and Infectious Diseases, by Cancer Center Support (CORE) Grant CA-21 765, and by American Lebanese Syrian Associated Charities. We thank Gary R. Burleson (U.S. Environmental Protection Agency) for help and advice regarding nonspecific immunity, and John Freeman and Tim Thomas for technical assistance.",

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Protection of chickens from lethal influenza virus infection by influenza A/chicken/Pennsylvania/1/83 virus: Characterization of the protective effect

Abstract

Bibliographical note

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