TY - JOUR
T1 - Protection of lung fibroblast MRC-5 cells against hydrogen peroxide-induced oxidative damage by 0.1-2.8 kDa antioxidative peptides isolated from whey protein hydrolysate
AU - Kong, Baohua
AU - Peng, Xinyan
AU - Xiong, Youling L.
AU - Zhao, Xinhuai
PY - 2012/11/15
Y1 - 2012/11/15
N2 - Antioxidative peptides (0.1-2.8 kDa) obtained from gel filtration of Alcalase-hydrolysed whey protein were subjected to individual peptide isolation by RP-HPLC. The sub-fraction P4 and a prominent pentapeptide identified by mass spectrometry (Val-His-Leu-Lys-Pro) (P4c) were found to be highly antioxidative, therefore, used to assess the efficacy against hydrogen peroxide (H 2O2)-induced human lung fibroblast MRC-5 cell oxidative injury. MRC-5 cells were damaged by incubation with H2O2, but cell death was significantly reduced when exposed to P4 and P4c (P < 0.05). Compared to the H2O2-damaged model control, P4 and P4c enhanced the activity of cell superoxide dismutase, glutathione peroxidase, and catalase, and decreased the levels of malonaldehyde (P < 0.05). These results, with a more pronounced action of P4c than P4, demonstrated that antioxidative peptides had a significant protection of MRC-5 cells against the toxicity caused by H2O2.
AB - Antioxidative peptides (0.1-2.8 kDa) obtained from gel filtration of Alcalase-hydrolysed whey protein were subjected to individual peptide isolation by RP-HPLC. The sub-fraction P4 and a prominent pentapeptide identified by mass spectrometry (Val-His-Leu-Lys-Pro) (P4c) were found to be highly antioxidative, therefore, used to assess the efficacy against hydrogen peroxide (H 2O2)-induced human lung fibroblast MRC-5 cell oxidative injury. MRC-5 cells were damaged by incubation with H2O2, but cell death was significantly reduced when exposed to P4 and P4c (P < 0.05). Compared to the H2O2-damaged model control, P4 and P4c enhanced the activity of cell superoxide dismutase, glutathione peroxidase, and catalase, and decreased the levels of malonaldehyde (P < 0.05). These results, with a more pronounced action of P4c than P4, demonstrated that antioxidative peptides had a significant protection of MRC-5 cells against the toxicity caused by H2O2.
KW - Antioxidant properties
KW - Hydrogen peroxide
KW - Lung fibroblast cell
KW - Whey protein hydrolysate
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U2 - 10.1016/j.foodchem.2012.04.122
DO - 10.1016/j.foodchem.2012.04.122
M3 - Article
C2 - 22868126
AN - SCOPUS:84863929753
VL - 135
SP - 540
EP - 547
IS - 2
ER -