Protein Kinase C mediated phosphorylation blocks juvenile hormone action

Damu R. Kethidi, Yiping Li, Subba R. Palli

Research output: Contribution to journalArticlepeer-review

23 Scopus citations


Juvenile hormones (JH) regulate a wide variety of developmental and physiological processes in insects. Although the biological actions of JH are well documented, the molecular mechanisms underlying JH action are poorly understood. We studied the molecular basis of JH action using a JH response element (JHRE) identified in the promoter region of JH esterase gene cloned from Choristoneura fumiferana, which is responsive to JH and 20-hydroxyecdysone (20E). In Drosophila melanogaster L57 cells, the JHRE-regulated reporter gene was induced by JH I, JH III, methoprene, and hydroprene. Nuclear proteins isolated from L57 cells bound to the JHRE and exposure of these proteins to ATP resulted in a reduction in their DNA binding. Either JH III or calf intestinal alkaline phosphatase (CIAP) was able to restore the binding of nuclear proteins to the DNA. In addition, protein kinase C inhibitors increased and protein kinase C activators reduced the binding of nuclear proteins to the JHRE. In transactivation assays, protein kinase C inhibitors induced the luciferase gene placed under the control of a minimal promoter and the JHRE. These data suggest that protein kinase C mediated phosphorylation prevents binding of nuclear proteins to juvenile hormone responsive promoters resulting in suppression of JH action.

Original languageEnglish
Pages (from-to)127-134
Number of pages8
JournalMolecular and Cellular Endocrinology
Issue number1-2
StatePublished - Mar 9 2006

Bibliographical note

Funding Information:
We would like to thank Drs. Peter and Lucy Cherbas of Indiana University for the gift of L57 cells. Supported by the NIH grant RO1 GM070559-01. This is contribution number 04-08-108 from the Kentucky Agricultural Experimental Station.


  • DNA-binding
  • Hormone action
  • Receptors
  • Response element
  • Signal transduction

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology


Dive into the research topics of 'Protein Kinase C mediated phosphorylation blocks juvenile hormone action'. Together they form a unique fingerprint.

Cite this