Protein levels and activity of some antioxidant enzymes in hippocampus of subjects with amnestic mild cognitive impairment

Rukhsana Sultana, Marta Piroddi, Francesco Galli, D. Allan Butterfield

Research output: Contribution to journalArticlepeer-review

102 Scopus citations

Abstract

Mild cognitive impairment (MCI) is generally referred to the transitional zone between normal cognitive aging and early dementia or clinically probable Alzheimer's disease (AD). Most individuals with amnestic MCI eventually develop AD, which suggests that MCI may be the earliest phase of AD. Oxidative stress is observed in brain from subjects with both AD and MCI. Among others, two possibilities for elevated oxidataive stress are decreased activity or elevated expression of antioxidant enzymes, the latter as a response to the former. Accordingly, in the current study, the protein levels and activity of some antioxidant enzymes in the hippocampus of control and MCI brain were measured using Western blot analysis and spectrophotometric methods, respectively. Alterations in the levels and activity of a number of antioxidant enzymes in MCI brain compared to age-matched controls were found. These results are consistent with the hypothesis that oxidative stress may be an early event in the progression of amnestic MCI to AD.

Original languageEnglish
Pages (from-to)2540-2546
Number of pages7
JournalNeurochemical Research
Volume33
Issue number12
DOIs
StatePublished - Dec 2008

Bibliographical note

Funding Information:
Acknowledgements The authors thank the faculty of the UK ADC for providing the brain specimens used for this study. This research was supported in part by NIH grants to D.A.B [AG-05119, AG-10836].

Keywords

  • Glucose-6-phosphate dehydrogenase
  • Glutathione
  • Glutathione peroxidise
  • Glutathione reductase
  • Hippocampus
  • Mild cognitive impairment
  • Oxidative stress
  • Superoxide dismutase

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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