Abstract
The effect of aging and diet restriction (DR) on the activity of creatine kinase (CK), glutamine synthetase (GS) and protein carbonyl formation in the cerebellum, hippocampus and cortex of male and female brown Norway (BN) rats has been investigated. It was demonstrated that CK activity in three different regions of the rat brain declines with age by 30%. Age-related decrease of GS activity was only 10-13% and did not reach statistical significance. Consistent with previously published studies, age-related increase of protein carbonyl content in each brain area studied has been observed. Preventive effects of a caloric restricted diet on the age-associated protein oxidation and changes of the activity of CK and GS in the brain was observed for both aging male and female BN rats. DR delayed the accumulation of protein carbonyls. Age-related changes of CK activity in rat brain were abrogated by DR. The activity of GS in the brain of old rats subjected to the caloric restricted diet was higher than that in the brain of young animals fed ad libitum. The results are consistent with the notion that DR may relieve age-associated level of oxidative stress and lessen protein damage.
Original language | English |
---|---|
Pages (from-to) | 157-168 |
Number of pages | 12 |
Journal | Mechanisms of Ageing and Development |
Volume | 100 |
Issue number | 2 |
DOIs | |
State | Published - Jan 30 1998 |
Bibliographical note
Funding Information:This work was supported in part by grants AG-10836 and AG-05119 from NIH.
Funding
This work was supported in part by grants AG-10836 and AG-05119 from NIH.
Funders | Funder number |
---|---|
National Institutes of Health (NIH) | |
National Institute on Aging | P01AG010836 |
Keywords
- Aging
- Creatine kinase
- Dietary restriction
- Glutamine synthetase
- Oxidative stress
- Protein oxidation
ASJC Scopus subject areas
- Aging
- Developmental Biology