Proteolytic activity, degradation, and dissolution of primary and permanent teeth

Debora Lopes Salles Scheffel, Jaime Aparecido Cury, Livia Maria Andaló Tenuta, Régis Henke Scheffel, Cristina Perez, Diana Gabriela Soares, Fernanda Gonçalves Basso, Carlos Alberto de Souza Costa, David Henry Pashley, Josimeri Hebling

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Background: Primary and permanent teeth composition may influence dissolution and degradation rates. Aim: To compare the dissolution and degradation of primary and permanent teeth. Design: Enamel and dentin powders were obtained from primary molars and premolars and incubated within different pH buffers. Calcium and inorganic phosphate release was quantified in the buffers by atomic absorption and light spectrophotometry. A colorimetric assay was used to assess the MMP activity of primary dentin (PrD) and permanent dentin (PeD). Collagen degradation was assessed by dry mass loss, change in elastic modulus (E), and ICTP and CTX release. Data were submitted to ANOVA and Tukey's tests (α = 0.05). Results: Similar dissolution was found between PrD and PeD after 256 hours. At pH 4.5, enamel released more minerals than dentin whereas at pH 5.5 the inverse result was observed. MMP activity was similar for both substrates. PrD showed higher dry mass loss after 1 week. In general, greater reduction in E was recorded for PrD. Higher quantities of ICTP and CTX were released from PrD after 1 week. Conclusions: Primary and permanent teeth presented similar demineralization rates. Collagen degradation, however, was faster and more substantial for PrD.

Original languageEnglish
Pages (from-to)650-659
Number of pages10
JournalInternational Journal of Paediatric Dentistry
Volume30
Issue number5
DOIs
StatePublished - Sep 1 2020

Bibliographical note

Publisher Copyright:
© 2020 BSPD, IAPD and John Wiley & Sons A/S. Published by John Wiley & Sons Ltd

Keywords

  • collagen
  • dentin
  • dissolution
  • enamel
  • proteases

ASJC Scopus subject areas

  • General Dentistry

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