TY - JOUR
T1 - Proteolytic activity, degradation, and dissolution of primary and permanent teeth
AU - Scheffel, Debora Lopes Salles
AU - Cury, Jaime Aparecido
AU - Tenuta, Livia Maria Andaló
AU - Scheffel, Régis Henke
AU - Perez, Cristina
AU - Soares, Diana Gabriela
AU - Basso, Fernanda Gonçalves
AU - Costa, Carlos Alberto de Souza
AU - Pashley, David Henry
AU - Hebling, Josimeri
N1 - Publisher Copyright:
© 2020 BSPD, IAPD and John Wiley & Sons A/S. Published by John Wiley & Sons Ltd
PY - 2020/9/1
Y1 - 2020/9/1
N2 - Background: Primary and permanent teeth composition may influence dissolution and degradation rates. Aim: To compare the dissolution and degradation of primary and permanent teeth. Design: Enamel and dentin powders were obtained from primary molars and premolars and incubated within different pH buffers. Calcium and inorganic phosphate release was quantified in the buffers by atomic absorption and light spectrophotometry. A colorimetric assay was used to assess the MMP activity of primary dentin (PrD) and permanent dentin (PeD). Collagen degradation was assessed by dry mass loss, change in elastic modulus (E), and ICTP and CTX release. Data were submitted to ANOVA and Tukey's tests (α = 0.05). Results: Similar dissolution was found between PrD and PeD after 256 hours. At pH 4.5, enamel released more minerals than dentin whereas at pH 5.5 the inverse result was observed. MMP activity was similar for both substrates. PrD showed higher dry mass loss after 1 week. In general, greater reduction in E was recorded for PrD. Higher quantities of ICTP and CTX were released from PrD after 1 week. Conclusions: Primary and permanent teeth presented similar demineralization rates. Collagen degradation, however, was faster and more substantial for PrD.
AB - Background: Primary and permanent teeth composition may influence dissolution and degradation rates. Aim: To compare the dissolution and degradation of primary and permanent teeth. Design: Enamel and dentin powders were obtained from primary molars and premolars and incubated within different pH buffers. Calcium and inorganic phosphate release was quantified in the buffers by atomic absorption and light spectrophotometry. A colorimetric assay was used to assess the MMP activity of primary dentin (PrD) and permanent dentin (PeD). Collagen degradation was assessed by dry mass loss, change in elastic modulus (E), and ICTP and CTX release. Data were submitted to ANOVA and Tukey's tests (α = 0.05). Results: Similar dissolution was found between PrD and PeD after 256 hours. At pH 4.5, enamel released more minerals than dentin whereas at pH 5.5 the inverse result was observed. MMP activity was similar for both substrates. PrD showed higher dry mass loss after 1 week. In general, greater reduction in E was recorded for PrD. Higher quantities of ICTP and CTX were released from PrD after 1 week. Conclusions: Primary and permanent teeth presented similar demineralization rates. Collagen degradation, however, was faster and more substantial for PrD.
KW - collagen
KW - dentin
KW - dissolution
KW - enamel
KW - proteases
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U2 - 10.1111/ipd.12632
DO - 10.1111/ipd.12632
M3 - Article
C2 - 32077547
AN - SCOPUS:85081252641
SN - 0960-7439
VL - 30
SP - 650
EP - 659
JO - International Journal of Paediatric Dentistry
JF - International Journal of Paediatric Dentistry
IS - 5
ER -