Proteolytic activity, degradation, and dissolution of primary and permanent teeth

Debora Lopes Salles Scheffel; Jaime Aparecido Cury; Livia Maria Andaló Tenuta; Régis Henke Scheffel; Cristina Perez; Diana Gabriela Soares; Fernanda Gonçalves Basso; Carlos Alberto de Souza Costa; David Henry Pashley; Josimeri Hebling

doi:10.1111/ipd.12632

Proteolytic activity, degradation, and dissolution of primary and permanent teeth

Debora Lopes Salles Scheffel
, Jaime Aparecido Cury
, Livia Maria Andaló Tenuta
, Régis Henke Scheffel
, Cristina Perez
, Diana Gabriela Soares
, Fernanda Gonçalves Basso
, Carlos Alberto de Souza Costa
, David Henry Pashley
, Josimeri Hebling

Oral Health Science

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Background: Primary and permanent teeth composition may influence dissolution and degradation rates. Aim: To compare the dissolution and degradation of primary and permanent teeth. Design: Enamel and dentin powders were obtained from primary molars and premolars and incubated within different pH buffers. Calcium and inorganic phosphate release was quantified in the buffers by atomic absorption and light spectrophotometry. A colorimetric assay was used to assess the MMP activity of primary dentin (PrD) and permanent dentin (PeD). Collagen degradation was assessed by dry mass loss, change in elastic modulus (E), and ICTP and CTX release. Data were submitted to ANOVA and Tukey's tests (α = 0.05). Results: Similar dissolution was found between PrD and PeD after 256 hours. At pH 4.5, enamel released more minerals than dentin whereas at pH 5.5 the inverse result was observed. MMP activity was similar for both substrates. PrD showed higher dry mass loss after 1 week. In general, greater reduction in E was recorded for PrD. Higher quantities of ICTP and CTX were released from PrD after 1 week. Conclusions: Primary and permanent teeth presented similar demineralization rates. Collagen degradation, however, was faster and more substantial for PrD.

Original language	English
Pages (from-to)	650-659
Number of pages	10
Journal	International Journal of Paediatric Dentistry
Volume	30
Issue number	5
DOIs	https://doi.org/10.1111/ipd.12632
State	Published - Sep 1 2020

Bibliographical note

Publisher Copyright:
© 2020 BSPD, IAPD and John Wiley & Sons A/S. Published by John Wiley & Sons Ltd

Funding

The authors thank Ms Kelli Ann Agee and Mr Waldomiro Vieira Filho for their valuable technical support. This work was supported in part by the S?o Paulo Research Foundation (FAPESP), grants 2008/02278-8; 2009/07924-8; 2014/17232-4 and 2014/18160-7, by Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior?Brasil (CAPES)? Finance Code 693711-0, and by R01 DE05306 from the National Institute of Dental and Craniofacial Research (NIDCR). The authors thank Ms Kelli Ann Agee and Mr Waldomiro Vieira Filho for their valuable technical support. This work was supported in part by the São Paulo Research Foundation (FAPESP), grants 2008/02278‐8; 2009/07924‐8; 2014/17232‐4 and 2014/18160‐7, by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brasil (CAPES)— Finance Code 693711‐0, and by R01 DE05306 from the National Institute of Dental and Craniofacial Research (NIDCR).

Funders	Funder number
S?o Paulo Research Foundation
National Institute of Dental and Craniofacial Research
Fundação de Amparo à Pesquisa do Estado de São Paulo	2008/02278‐8, 2009/07924‐8, 2014/17232‐4, 2014/18160‐7
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior	R01 DE05306

Keywords

collagen
dentin
dissolution
enamel
proteases

ASJC Scopus subject areas

General Dentistry

Access to Document

10.1111/ipd.12632

Cite this

@article{0fdae9b2a3ca4de5a13b8139cd2da084,

title = "Proteolytic activity, degradation, and dissolution of primary and permanent teeth",

abstract = "Background: Primary and permanent teeth composition may influence dissolution and degradation rates. Aim: To compare the dissolution and degradation of primary and permanent teeth. Design: Enamel and dentin powders were obtained from primary molars and premolars and incubated within different pH buffers. Calcium and inorganic phosphate release was quantified in the buffers by atomic absorption and light spectrophotometry. A colorimetric assay was used to assess the MMP activity of primary dentin (PrD) and permanent dentin (PeD). Collagen degradation was assessed by dry mass loss, change in elastic modulus (E), and ICTP and CTX release. Data were submitted to ANOVA and Tukey's tests (α = 0.05). Results: Similar dissolution was found between PrD and PeD after 256 hours. At pH 4.5, enamel released more minerals than dentin whereas at pH 5.5 the inverse result was observed. MMP activity was similar for both substrates. PrD showed higher dry mass loss after 1 week. In general, greater reduction in E was recorded for PrD. Higher quantities of ICTP and CTX were released from PrD after 1 week. Conclusions: Primary and permanent teeth presented similar demineralization rates. Collagen degradation, however, was faster and more substantial for PrD.",

keywords = "collagen, dentin, dissolution, enamel, proteases",

author = "Scheffel, \{Debora Lopes Salles\} and Cury, \{Jaime Aparecido\} and Tenuta, \{Livia Maria Andal{\'o}\} and Scheffel, \{R{\'e}gis Henke\} and Cristina Perez and Soares, \{Diana Gabriela\} and Basso, \{Fernanda Gon{\c c}alves\} and Costa, \{Carlos Alberto de Souza\} and Pashley, \{David Henry\} and Josimeri Hebling",

note = "Publisher Copyright: {\textcopyright} 2020 BSPD, IAPD and John Wiley \& Sons A/S. Published by John Wiley \& Sons Ltd",

year = "2020",

month = sep,

day = "1",

doi = "10.1111/ipd.12632",

language = "English",

volume = "30",

pages = "650--659",

number = "5",

}

TY - JOUR

T1 - Proteolytic activity, degradation, and dissolution of primary and permanent teeth

AU - Scheffel, Debora Lopes Salles

AU - Cury, Jaime Aparecido

AU - Tenuta, Livia Maria Andaló

AU - Scheffel, Régis Henke

AU - Perez, Cristina

AU - Soares, Diana Gabriela

AU - Basso, Fernanda Gonçalves

AU - Costa, Carlos Alberto de Souza

AU - Pashley, David Henry

AU - Hebling, Josimeri

PY - 2020/9/1

Y1 - 2020/9/1

N2 - Background: Primary and permanent teeth composition may influence dissolution and degradation rates. Aim: To compare the dissolution and degradation of primary and permanent teeth. Design: Enamel and dentin powders were obtained from primary molars and premolars and incubated within different pH buffers. Calcium and inorganic phosphate release was quantified in the buffers by atomic absorption and light spectrophotometry. A colorimetric assay was used to assess the MMP activity of primary dentin (PrD) and permanent dentin (PeD). Collagen degradation was assessed by dry mass loss, change in elastic modulus (E), and ICTP and CTX release. Data were submitted to ANOVA and Tukey's tests (α = 0.05). Results: Similar dissolution was found between PrD and PeD after 256 hours. At pH 4.5, enamel released more minerals than dentin whereas at pH 5.5 the inverse result was observed. MMP activity was similar for both substrates. PrD showed higher dry mass loss after 1 week. In general, greater reduction in E was recorded for PrD. Higher quantities of ICTP and CTX were released from PrD after 1 week. Conclusions: Primary and permanent teeth presented similar demineralization rates. Collagen degradation, however, was faster and more substantial for PrD.

AB - Background: Primary and permanent teeth composition may influence dissolution and degradation rates. Aim: To compare the dissolution and degradation of primary and permanent teeth. Design: Enamel and dentin powders were obtained from primary molars and premolars and incubated within different pH buffers. Calcium and inorganic phosphate release was quantified in the buffers by atomic absorption and light spectrophotometry. A colorimetric assay was used to assess the MMP activity of primary dentin (PrD) and permanent dentin (PeD). Collagen degradation was assessed by dry mass loss, change in elastic modulus (E), and ICTP and CTX release. Data were submitted to ANOVA and Tukey's tests (α = 0.05). Results: Similar dissolution was found between PrD and PeD after 256 hours. At pH 4.5, enamel released more minerals than dentin whereas at pH 5.5 the inverse result was observed. MMP activity was similar for both substrates. PrD showed higher dry mass loss after 1 week. In general, greater reduction in E was recorded for PrD. Higher quantities of ICTP and CTX were released from PrD after 1 week. Conclusions: Primary and permanent teeth presented similar demineralization rates. Collagen degradation, however, was faster and more substantial for PrD.

KW - collagen

KW - dentin

KW - dissolution

KW - enamel

KW - proteases

UR - https://www.scopus.com/pages/publications/85081252641

UR - https://www.scopus.com/pages/publications/85081252641#tab=citedBy

U2 - 10.1111/ipd.12632

DO - 10.1111/ipd.12632

M3 - Article

C2 - 32077547

AN - SCOPUS:85081252641

SN - 0960-7439

VL - 30

SP - 650

EP - 659

JO - International Journal of Paediatric Dentistry

JF - International Journal of Paediatric Dentistry

IS - 5

ER -

Proteolytic activity, degradation, and dissolution of primary and permanent teeth

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this