TY - JOUR
T1 - Proteolytic degradation of the flavonoid regulators, TRANSPARENT TESTA8 and TRANSPARENT TESTA GLABRA1, in Arabidopsis is mediated by the ubiquitin/26sproteasome system
AU - Patra, Barunava
AU - Pattanaik, Sitakanta
AU - Yuan, Ling
N1 - Funding Information:
This work is supported by a grant from the Kentucky Tobacco Research and Development Center (to LY). We thank KA Shen for critical comments of the manuscript.
PY - 2013/10
Y1 - 2013/10
N2 - Regulated proteolysis by the ubiquitin/26S proteasome system (uPS) has emerged as a major posttranslational control mechanism regulating transcription factor (tF) activity in plants. anthocyanin biosynthesis in Arabidopsis is regulated by a ternary complex comprised of basic helix-loop-helix (bhLh), r2r3mYB and WD-repeat (WDr) proteins. the bhLh TF, TRANSPARENT TESTA 8 (tt8), and the WDr protein, tranSParEnt tESta GLaBra1 (ttG1), are essential for expression of late flavonoid biosynthesis genes. Previous studies have demonstrated that the turnover of several anthocyanin pathway regulators is controlled by the uPS. here, we show that tt8 and ttG1 are short-lived and targeted by the uPS for degradation. our findings further extend our understanding of the role of the uPS in the regulation of anthocyanin biosynthesis in plants.
AB - Regulated proteolysis by the ubiquitin/26S proteasome system (uPS) has emerged as a major posttranslational control mechanism regulating transcription factor (tF) activity in plants. anthocyanin biosynthesis in Arabidopsis is regulated by a ternary complex comprised of basic helix-loop-helix (bhLh), r2r3mYB and WD-repeat (WDr) proteins. the bhLh TF, TRANSPARENT TESTA 8 (tt8), and the WDr protein, tranSParEnt tESta GLaBra1 (ttG1), are essential for expression of late flavonoid biosynthesis genes. Previous studies have demonstrated that the turnover of several anthocyanin pathway regulators is controlled by the uPS. here, we show that tt8 and ttG1 are short-lived and targeted by the uPS for degradation. our findings further extend our understanding of the role of the uPS in the regulation of anthocyanin biosynthesis in plants.
KW - 26S proteasome
KW - Anthocyanins
KW - Flavonoids
KW - TT8
KW - TTG1
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U2 - 10.4161/psb.25901
DO - 10.4161/psb.25901
M3 - Article
C2 - 23921543
AN - SCOPUS:84885220979
SN - 1559-2316
VL - 8
JO - Plant Signaling and Behavior
JF - Plant Signaling and Behavior
IS - 10
ER -