Proteolytic degradation of the flavonoid regulators, TRANSPARENT TESTA8 and TRANSPARENT TESTA GLABRA1, in Arabidopsis is mediated by the ubiquitin/26sproteasome system

Barunava Patra; Sitakanta Pattanaik; Ling Yuan

doi:10.4161/psb.25901

Proteolytic degradation of the flavonoid regulators, TRANSPARENT TESTA8 and TRANSPARENT TESTA GLABRA1, in Arabidopsis is mediated by the ubiquitin/26sproteasome system

Barunava Patra, Sitakanta Pattanaik, Ling Yuan

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Regulated proteolysis by the ubiquitin/26S proteasome system (uPS) has emerged as a major posttranslational control mechanism regulating transcription factor (tF) activity in plants. anthocyanin biosynthesis in Arabidopsis is regulated by a ternary complex comprised of basic helix-loop-helix (bhLh), r2r3mYB and WD-repeat (WDr) proteins. the bhLh TF, TRANSPARENT TESTA 8 (tt8), and the WDr protein, tranSParEnt tESta GLaBra1 (ttG1), are essential for expression of late flavonoid biosynthesis genes. Previous studies have demonstrated that the turnover of several anthocyanin pathway regulators is controlled by the uPS. here, we show that tt8 and ttG1 are short-lived and targeted by the uPS for degradation. our findings further extend our understanding of the role of the uPS in the regulation of anthocyanin biosynthesis in plants.

Original language	English
Journal	Plant Signaling and Behavior
Volume	8
Issue number	10
DOIs	https://doi.org/10.4161/psb.25901
State	Published - Oct 2013

Bibliographical note

Funding Information:
This work is supported by a grant from the Kentucky Tobacco Research and Development Center (to LY). We thank KA Shen for critical comments of the manuscript.

Funding

This work is supported by a grant from the Kentucky Tobacco Research and Development Center (to LY). We thank KA Shen for critical comments of the manuscript.

Funders	Funder number
The Kentucky Tobacco Research and Development Center

Keywords

26S proteasome
Anthocyanins
Flavonoids
TT8
TTG1

ASJC Scopus subject areas

Plant Science

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10.4161/psb.25901

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title = "Proteolytic degradation of the flavonoid regulators, TRANSPARENT TESTA8 and TRANSPARENT TESTA GLABRA1, in Arabidopsis is mediated by the ubiquitin/26sproteasome system",

abstract = "Regulated proteolysis by the ubiquitin/26S proteasome system (uPS) has emerged as a major posttranslational control mechanism regulating transcription factor (tF) activity in plants. anthocyanin biosynthesis in Arabidopsis is regulated by a ternary complex comprised of basic helix-loop-helix (bhLh), r2r3mYB and WD-repeat (WDr) proteins. the bhLh TF, TRANSPARENT TESTA 8 (tt8), and the WDr protein, tranSParEnt tESta GLaBra1 (ttG1), are essential for expression of late flavonoid biosynthesis genes. Previous studies have demonstrated that the turnover of several anthocyanin pathway regulators is controlled by the uPS. here, we show that tt8 and ttG1 are short-lived and targeted by the uPS for degradation. our findings further extend our understanding of the role of the uPS in the regulation of anthocyanin biosynthesis in plants.",

keywords = "26S proteasome, Anthocyanins, Flavonoids, TT8, TTG1",

author = "Barunava Patra and Sitakanta Pattanaik and Ling Yuan",

note = "Funding Information: This work is supported by a grant from the Kentucky Tobacco Research and Development Center (to LY). We thank KA Shen for critical comments of the manuscript.",

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doi = "10.4161/psb.25901",

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AU - Patra, Barunava

AU - Pattanaik, Sitakanta

AU - Yuan, Ling

N1 - Funding Information: This work is supported by a grant from the Kentucky Tobacco Research and Development Center (to LY). We thank KA Shen for critical comments of the manuscript.

PY - 2013/10

Y1 - 2013/10

N2 - Regulated proteolysis by the ubiquitin/26S proteasome system (uPS) has emerged as a major posttranslational control mechanism regulating transcription factor (tF) activity in plants. anthocyanin biosynthesis in Arabidopsis is regulated by a ternary complex comprised of basic helix-loop-helix (bhLh), r2r3mYB and WD-repeat (WDr) proteins. the bhLh TF, TRANSPARENT TESTA 8 (tt8), and the WDr protein, tranSParEnt tESta GLaBra1 (ttG1), are essential for expression of late flavonoid biosynthesis genes. Previous studies have demonstrated that the turnover of several anthocyanin pathway regulators is controlled by the uPS. here, we show that tt8 and ttG1 are short-lived and targeted by the uPS for degradation. our findings further extend our understanding of the role of the uPS in the regulation of anthocyanin biosynthesis in plants.

AB - Regulated proteolysis by the ubiquitin/26S proteasome system (uPS) has emerged as a major posttranslational control mechanism regulating transcription factor (tF) activity in plants. anthocyanin biosynthesis in Arabidopsis is regulated by a ternary complex comprised of basic helix-loop-helix (bhLh), r2r3mYB and WD-repeat (WDr) proteins. the bhLh TF, TRANSPARENT TESTA 8 (tt8), and the WDr protein, tranSParEnt tESta GLaBra1 (ttG1), are essential for expression of late flavonoid biosynthesis genes. Previous studies have demonstrated that the turnover of several anthocyanin pathway regulators is controlled by the uPS. here, we show that tt8 and ttG1 are short-lived and targeted by the uPS for degradation. our findings further extend our understanding of the role of the uPS in the regulation of anthocyanin biosynthesis in plants.

KW - 26S proteasome

KW - Anthocyanins

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KW - TT8

KW - TTG1

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SN - 1559-2316

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JO - Plant Signaling and Behavior

JF - Plant Signaling and Behavior

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Proteolytic degradation of the flavonoid regulators, TRANSPARENT TESTA8 and TRANSPARENT TESTA GLABRA1, in Arabidopsis is mediated by the ubiquitin/26sproteasome system

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

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