Proteomic characterization of equine cerebrospinal fluid

Carolyn J. Broccardo, Gisela Soboll Hussey, Lutz Goehring, Paul Lunn, Jessica E. Prenni

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Cerebrospinal fluid (CSF) is a biofluid that is reflective of overall health. Although proteomic profiling of human CSF has been performed in the context of a variety of disease states, this report represents the first comprehensive proteomic analysis of equine CSF. A total of 320 proteins were confidently identified across six healthy horses, and these proteins were further characterized by gene ontology terms mapped in UniProt, and normalized spectral abundance factors were calculated as a measure of relative abundance. Theses results provide an optimized protocol for analysis of equine CSF and lay the groundwork for future studies involving the study of equine CSF in the context of pathogenic disease states.

Original languageEnglish
Pages (from-to)451-458
Number of pages8
JournalJournal of Equine Veterinary Science
Volume34
Issue number3
DOIs
StatePublished - Mar 2014

Bibliographical note

Funding Information:
This research was supported in part by the College Research Council, the Infectious Disease Research Cluster of Colorado State University, and the Biotechnology Research and Development Corporation. Mass spectrometry proteomics data were deposited in the ProteomeXchange Consortium database ( http://proteomecentral.proteomexchange.orgs ) via the PRIDE partner repository with the dataset identifier PXD000133. All mass spectrometry was performed in the Proteomics and Metabolomics Facility at Colorado State University ( www.pmf.colostate.edu ).

Keywords

  • Cerebrospinal fluid
  • Equine
  • Gene ontology
  • Normalized spectral abundance factor
  • Proteomics

ASJC Scopus subject areas

  • Equine

Fingerprint

Dive into the research topics of 'Proteomic characterization of equine cerebrospinal fluid'. Together they form a unique fingerprint.

Cite this