TY - JOUR
T1 - Protocol for optimizing production and quality control of infective EcoHIV virions
AU - Alfar, Hammodah R.
AU - Pariser, Daphne N.
AU - Chanzu, Harry
AU - Joshi, Smita
AU - Coenen, Daniëlle M.
AU - Lykins, Joshua
AU - Prakhya, Kanakanagavalli Shravani
AU - Potash, Mary Jane
AU - Chao, Wei
AU - Kelschenbach, Jennifer
AU - Volsky, David J.
AU - Metcalf-Pate, Kelly
AU - Whiteheart, Sidney W.
N1 - Publisher Copyright:
© 2023 The Authors
PY - 2023/9/15
Y1 - 2023/9/15
N2 - EcoHIV is a model of HIV infection that recapitulates aspects of HIV-1 pathology in mice. However, there are limited published protocols to guide EcoHIV virion production. Here, we present a protocol for producing infective EcoHIV virions and essential quality controls. We describe steps for viral purification, titering, and multiple techniques to analyze infection efficacy. This protocol produces high infectivity in C57BL/6 mice which will aid investigators in generating preclinical data.
AB - EcoHIV is a model of HIV infection that recapitulates aspects of HIV-1 pathology in mice. However, there are limited published protocols to guide EcoHIV virion production. Here, we present a protocol for producing infective EcoHIV virions and essential quality controls. We describe steps for viral purification, titering, and multiple techniques to analyze infection efficacy. This protocol produces high infectivity in C57BL/6 mice which will aid investigators in generating preclinical data.
KW - Cell Culture
KW - Cell-based Assays
KW - Health Sciences
UR - http://www.scopus.com/inward/record.url?scp=85162181690&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85162181690&partnerID=8YFLogxK
U2 - 10.1016/j.xpro.2023.102368
DO - 10.1016/j.xpro.2023.102368
M3 - Article
C2 - 37342907
AN - SCOPUS:85162181690
VL - 4
JO - STAR Protocols
JF - STAR Protocols
IS - 3
M1 - 102368
ER -
