Protocol for optimizing production and quality control of infective EcoHIV virions

Hammodah R. Alfar; Daphne N. Pariser; Harry Chanzu; Smita Joshi; Daniëlle M. Coenen; Joshua Lykins; Kanakanagavalli Shravani Prakhya; Mary Jane Potash; Wei Chao; Jennifer Kelschenbach; David J. Volsky; Kelly Metcalf-Pate; Sidney W. Whiteheart

doi:10.1016/j.xpro.2023.102368

Protocol for optimizing production and quality control of infective EcoHIV virions

Hammodah R. Alfar, Daphne N. Pariser, Harry Chanzu, Smita Joshi, Daniëlle M. Coenen, Joshua Lykins, Kanakanagavalli Shravani Prakhya, Mary Jane Potash, Wei Chao, Jennifer Kelschenbach, David J. Volsky, Kelly Metcalf-Pate, Sidney W. Whiteheart

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

EcoHIV is a model of HIV infection that recapitulates aspects of HIV-1 pathology in mice. However, there are limited published protocols to guide EcoHIV virion production. Here, we present a protocol for producing infective EcoHIV virions and essential quality controls. We describe steps for viral purification, titering, and multiple techniques to analyze infection efficacy. This protocol produces high infectivity in C57BL/6 mice which will aid investigators in generating preclinical data.

Original language	English
Article number	102368
Journal	STAR Protocols
Volume	4
Issue number	3
DOIs	https://doi.org/10.1016/j.xpro.2023.102368
State	Published - Sep 15 2023

Bibliographical note

Publisher Copyright:
© 2023 The Authors

Funding

The authors thank the members of the Whiteheart laboratory for their careful perusal of the manuscript. We also thank Kearstin Edmonds from the laboratory of Dr. Rebecca Dutch for her help and suggestions during our troubleshooting experiments. This work is supported by grants from the National Institutes of Health , National Heart, Lung, and Blood Institute ( HL56652 , HL138179 , and HL150818 to S.W.W.).

Funders	Funder number
National Institutes of Health (NIH)
National Heart, Lung, and Blood Institute (NHLBI)	HL138179, HL150818, HL56652
National Heart, Lung, and Blood Institute (NHLBI)

Keywords

Cell Culture
Cell-based Assays
Health Sciences

ASJC Scopus subject areas

General Neuroscience
General Biochemistry, Genetics and Molecular Biology
General Immunology and Microbiology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.xpro.2023.102368

Cite this

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abstract = "EcoHIV is a model of HIV infection that recapitulates aspects of HIV-1 pathology in mice. However, there are limited published protocols to guide EcoHIV virion production. Here, we present a protocol for producing infective EcoHIV virions and essential quality controls. We describe steps for viral purification, titering, and multiple techniques to analyze infection efficacy. This protocol produces high infectivity in C57BL/6 mice which will aid investigators in generating preclinical data.",

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AU - Alfar, Hammodah R.

AU - Pariser, Daphne N.

AU - Chanzu, Harry

AU - Joshi, Smita

AU - Coenen, Daniëlle M.

AU - Lykins, Joshua

AU - Prakhya, Kanakanagavalli Shravani

AU - Potash, Mary Jane

AU - Chao, Wei

AU - Kelschenbach, Jennifer

AU - Volsky, David J.

AU - Metcalf-Pate, Kelly

AU - Whiteheart, Sidney W.

PY - 2023/9/15

Y1 - 2023/9/15

N2 - EcoHIV is a model of HIV infection that recapitulates aspects of HIV-1 pathology in mice. However, there are limited published protocols to guide EcoHIV virion production. Here, we present a protocol for producing infective EcoHIV virions and essential quality controls. We describe steps for viral purification, titering, and multiple techniques to analyze infection efficacy. This protocol produces high infectivity in C57BL/6 mice which will aid investigators in generating preclinical data.

AB - EcoHIV is a model of HIV infection that recapitulates aspects of HIV-1 pathology in mice. However, there are limited published protocols to guide EcoHIV virion production. Here, we present a protocol for producing infective EcoHIV virions and essential quality controls. We describe steps for viral purification, titering, and multiple techniques to analyze infection efficacy. This protocol produces high infectivity in C57BL/6 mice which will aid investigators in generating preclinical data.

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KW - Cell-based Assays

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Protocol for optimizing production and quality control of infective EcoHIV virions

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

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