Protocol for rapid assessment of the efficacy of novel Wnt inhibitors using zebrafish models

Meghan G. Haney; Mary Wimsett; Chunming Liu; Jessica S. Blackburn

doi:10.1016/j.xpro.2021.100433

Protocol for rapid assessment of the efficacy of novel Wnt inhibitors using zebrafish models

Meghan G. Haney, Mary Wimsett, Chunming Liu, Jessica S. Blackburn

Molecular and Cellular Biochemistry

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Dysregulation of Wnt signaling is a hallmark of many cancers, and the development of effective, non-toxic small-molecule Wnt inhibitors is desirable. Off-target toxicities of new compounds are typically tested in mouse models, which is both costly and time consuming. Here, we present a rapid and inexpensive protocol to determine the in vivo toxicity and efficacy of novel Wnt inhibitors in zebrafish using a combination of a fluorescence reporter assay as well as eye rescue and fin regeneration assays. These experiments are completed within 1 week to rapidly narrow drug candidates before moving to more expensive pre-clinical testing. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2020).

Original language	English
Article number	100433
Journal	STAR Protocols
Volume	2
Issue number	2
DOIs	https://doi.org/10.1016/j.xpro.2021.100433
State	Published - Jun 18 2021

Bibliographical note

Funding Information:
This research was funded by a V Foundation V Scholar Award and NIH Grant DP2CA228043 (to J.S.B.) and NIH Training Grant T32CA165990 (to M.G.H.). The graphical abstract was made using Biorender.com. Conceptualization, M.G.H. J.S.B. and C.L.; investigation, M.G.H.; writing – original draft, M.W. and M.G.H.; writing – review & editing, M.W. M.G.H. J.S.B. and C.L.; funding acquisition, M.G.H. and J.S.B.; supervision, J.S.B. The authors declare no competing interests.

Publisher Copyright:
© 2021 The Authors

Keywords

Cancer
High Throughput Screening
Model Organisms
Signal Transduction

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology (all)
Neuroscience (all)
Immunology and Microbiology (all)
Medicine (all)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.xpro.2021.100433

Cite this

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abstract = "Dysregulation of Wnt signaling is a hallmark of many cancers, and the development of effective, non-toxic small-molecule Wnt inhibitors is desirable. Off-target toxicities of new compounds are typically tested in mouse models, which is both costly and time consuming. Here, we present a rapid and inexpensive protocol to determine the in vivo toxicity and efficacy of novel Wnt inhibitors in zebrafish using a combination of a fluorescence reporter assay as well as eye rescue and fin regeneration assays. These experiments are completed within 1 week to rapidly narrow drug candidates before moving to more expensive pre-clinical testing. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2020).",

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AU - Blackburn, Jessica S.

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AB - Dysregulation of Wnt signaling is a hallmark of many cancers, and the development of effective, non-toxic small-molecule Wnt inhibitors is desirable. Off-target toxicities of new compounds are typically tested in mouse models, which is both costly and time consuming. Here, we present a rapid and inexpensive protocol to determine the in vivo toxicity and efficacy of novel Wnt inhibitors in zebrafish using a combination of a fluorescence reporter assay as well as eye rescue and fin regeneration assays. These experiments are completed within 1 week to rapidly narrow drug candidates before moving to more expensive pre-clinical testing. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2020).

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KW - High Throughput Screening

KW - Model Organisms

KW - Signal Transduction

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Protocol for rapid assessment of the efficacy of novel Wnt inhibitors using zebrafish models

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

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