Protocol for rapid assessment of the efficacy of novel Wnt inhibitors using zebrafish models

Meghan G. Haney, Mary Wimsett, Chunming Liu, Jessica S. Blackburn

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


Dysregulation of Wnt signaling is a hallmark of many cancers, and the development of effective, non-toxic small-molecule Wnt inhibitors is desirable. Off-target toxicities of new compounds are typically tested in mouse models, which is both costly and time consuming. Here, we present a rapid and inexpensive protocol to determine the in vivo toxicity and efficacy of novel Wnt inhibitors in zebrafish using a combination of a fluorescence reporter assay as well as eye rescue and fin regeneration assays. These experiments are completed within 1 week to rapidly narrow drug candidates before moving to more expensive pre-clinical testing. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2020).

Original languageEnglish
Article number100433
JournalSTAR Protocols
Issue number2
StatePublished - Jun 18 2021

Bibliographical note

Funding Information:
This research was funded by a V Foundation V Scholar Award and NIH Grant DP2CA228043 (to J.S.B.) and NIH Training Grant T32CA165990 (to M.G.H.). The graphical abstract was made using Conceptualization, M.G.H. J.S.B. and C.L.; investigation, M.G.H.; writing – original draft, M.W. and M.G.H.; writing – review & editing, M.W. M.G.H. J.S.B. and C.L.; funding acquisition, M.G.H. and J.S.B.; supervision, J.S.B. The authors declare no competing interests.

Publisher Copyright:
© 2021 The Authors


  • Cancer
  • High Throughput Screening
  • Model Organisms
  • Signal Transduction

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology (all)
  • Neuroscience (all)
  • Immunology and Microbiology (all)
  • Medicine (all)


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