Abstract
Dysregulation of Wnt signaling is a hallmark of many cancers, and the development of effective, non-toxic small-molecule Wnt inhibitors is desirable. Off-target toxicities of new compounds are typically tested in mouse models, which is both costly and time consuming. Here, we present a rapid and inexpensive protocol to determine the in vivo toxicity and efficacy of novel Wnt inhibitors in zebrafish using a combination of a fluorescence reporter assay as well as eye rescue and fin regeneration assays. These experiments are completed within 1 week to rapidly narrow drug candidates before moving to more expensive pre-clinical testing. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2020).
| Original language | English |
|---|---|
| Article number | 100433 |
| Journal | STAR Protocols |
| Volume | 2 |
| Issue number | 2 |
| DOIs | |
| State | Published - Jun 18 2021 |
Bibliographical note
Publisher Copyright:© 2021 The Authors
Funding
This research was funded by a V Foundation V Scholar Award and NIH Grant DP2CA228043 (to J.S.B.) and NIH Training Grant T32CA165990 (to M.G.H.). The graphical abstract was made using Biorender.com. Conceptualization, M.G.H. J.S.B. and C.L.; investigation, M.G.H.; writing – original draft, M.W. and M.G.H.; writing – review & editing, M.W. M.G.H. J.S.B. and C.L.; funding acquisition, M.G.H. and J.S.B.; supervision, J.S.B. The authors declare no competing interests.
| Funders | Funder number |
|---|---|
| National Institutes of Health (NIH) | DP2CA228043, T32CA165990 |
| National Childhood Cancer Registry – National Cancer Institute | R37CA227656 |
| V Foundation for Cancer Research |
Keywords
- Cancer
- High Throughput Screening
- Model Organisms
- Signal Transduction
ASJC Scopus subject areas
- General Neuroscience
- General Biochemistry, Genetics and Molecular Biology
- General Immunology and Microbiology
