Proximate composition of enhanced DGAT high oil, high protein soybeans

Maythem AL-Amery; Bruce Downie; Seth DeBolt; Mark Crocker; Kristine Urschel; Ben Goff; Nicholas Teets; Jarrad Gollihue; David Hildebrand

doi:10.1016/j.bcab.2019.101303

Proximate composition of enhanced DGAT high oil, high protein soybeans

Maythem AL-Amery, Bruce Downie, Seth DeBolt, Mark Crocker, Kristine Urschel, Ben Goff, Nicholas Teets, Jarrad Gollihue, David Hildebrand

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Developing new soybean (Glycine max) varieties with greater amounts of oil and protein enhances soybean as a renewable source of food and fuel. Soybean expressing highly active ACYL-COA: Diacylglycerol acyltransferase (DGAT) from Vernonia galamensis had oil increased by 4% with no protein reduction. To assess other seed composition changes, seeds of two independent transgenic (VgD) soybean lines and controls were obtained from two field locations in multiple years. Many analyses addressed what seed component(s) was/were reduced to compensate for the oil increase, including carbohydrate analysis (structural and nonstructural), NDF (Neutral detergent fiber), main minerals and proximate analyses. In Princeton, oil increased by 4% for the high oil lines in 2016 and 2017, with no significant reduction in protein, this was slightly different in Spindletop with an increase in oil by 1.6–1.7 %, and an increase in protein by 2.6–2.4 % in comparison to the control, as an average for both high oil lines in 2016 and 2017. Soluble carbohydrate was reduced in VgD by 1.2 % in Princeton location for the high oil lines in 2016 and 2017, whereas only VgD1-2 was significantly reduced in Spindletop in comparison to Jack on 2016 with 2%, Oil in the VgD seeds was greater than in control seeds without a decrease in seed protein in both locations, suggesting that the trait was maintained across environments.

Original language	English
Article number	101303
Journal	Biocatalysis and Agricultural Biotechnology
Volume	21
DOIs	https://doi.org/10.1016/j.bcab.2019.101303
State	Published - Sep 2019

Bibliographical note

Funding Information:
We would like to thank (HCED) The Committee for Higher Education and Development in IRAQ for their support. Jack (wild type) seed was provided by Dr. Brian Diers, Department of Crop Sciences, University of Illinois, Urbana, IL. At the University of Kentucky, we would like to thank Kai Su (Plant and Soil Science Department), Tonya Morgan (Center for Applied Energy Research), Tammy Barnes, (Department of Animal and Food Science), John Johnson and James Crutchfield for their help and support. Additional support from the Kentucky Agricultural Experiment Station.

Funding Information:
We would like to thank (HCED) The Committee for Higher Education and Development in IRAQ for their support. Jack (wild type) seed was provided by Dr. Brian Diers, Department of Crop Sciences, University of Illinois, Urbana, IL. At the University of Kentucky, we would like to thank Kai Su (Plant and Soil Science Department), Tonya Morgan (Center for Applied Energy Research), Tammy Barnes, (Department of Animal and Food Science), John Johnson and James Crutchfield for their help and support. Additional support from the Kentucky Agricultural Experiment Station .

Publisher Copyright:
© 2019

Keywords

Glycine max
Oilseeds
Protein meal
Proximate analysis
Renewable oil
Seed composition

ASJC Scopus subject areas

Biotechnology
Food Science
Bioengineering
Applied Microbiology and Biotechnology
Agronomy and Crop Science

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.bcab.2019.101303

Cite this

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title = "Proximate composition of enhanced DGAT high oil, high protein soybeans",

abstract = "Developing new soybean (Glycine max) varieties with greater amounts of oil and protein enhances soybean as a renewable source of food and fuel. Soybean expressing highly active ACYL-COA: Diacylglycerol acyltransferase (DGAT) from Vernonia galamensis had oil increased by 4% with no protein reduction. To assess other seed composition changes, seeds of two independent transgenic (VgD) soybean lines and controls were obtained from two field locations in multiple years. Many analyses addressed what seed component(s) was/were reduced to compensate for the oil increase, including carbohydrate analysis (structural and nonstructural), NDF (Neutral detergent fiber), main minerals and proximate analyses. In Princeton, oil increased by 4% for the high oil lines in 2016 and 2017, with no significant reduction in protein, this was slightly different in Spindletop with an increase in oil by 1.6–1.7 %, and an increase in protein by 2.6–2.4 % in comparison to the control, as an average for both high oil lines in 2016 and 2017. Soluble carbohydrate was reduced in VgD by 1.2 % in Princeton location for the high oil lines in 2016 and 2017, whereas only VgD1-2 was significantly reduced in Spindletop in comparison to Jack on 2016 with 2%, Oil in the VgD seeds was greater than in control seeds without a decrease in seed protein in both locations, suggesting that the trait was maintained across environments.",

keywords = "Glycine max, Oilseeds, Protein meal, Proximate analysis, Renewable oil, Seed composition",

author = "Maythem AL-Amery and Bruce Downie and Seth DeBolt and Mark Crocker and Kristine Urschel and Ben Goff and Nicholas Teets and Jarrad Gollihue and David Hildebrand",

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AU - Downie, Bruce

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AU - Crocker, Mark

AU - Urschel, Kristine

AU - Goff, Ben

AU - Teets, Nicholas

AU - Gollihue, Jarrad

AU - Hildebrand, David

N1 - Funding Information: We would like to thank (HCED) The Committee for Higher Education and Development in IRAQ for their support. Jack (wild type) seed was provided by Dr. Brian Diers, Department of Crop Sciences, University of Illinois, Urbana, IL. At the University of Kentucky, we would like to thank Kai Su (Plant and Soil Science Department), Tonya Morgan (Center for Applied Energy Research), Tammy Barnes, (Department of Animal and Food Science), John Johnson and James Crutchfield for their help and support. Additional support from the Kentucky Agricultural Experiment Station. Funding Information: We would like to thank (HCED) The Committee for Higher Education and Development in IRAQ for their support. Jack (wild type) seed was provided by Dr. Brian Diers, Department of Crop Sciences, University of Illinois, Urbana, IL. At the University of Kentucky, we would like to thank Kai Su (Plant and Soil Science Department), Tonya Morgan (Center for Applied Energy Research), Tammy Barnes, (Department of Animal and Food Science), John Johnson and James Crutchfield for their help and support. Additional support from the Kentucky Agricultural Experiment Station . Publisher Copyright: © 2019

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AB - Developing new soybean (Glycine max) varieties with greater amounts of oil and protein enhances soybean as a renewable source of food and fuel. Soybean expressing highly active ACYL-COA: Diacylglycerol acyltransferase (DGAT) from Vernonia galamensis had oil increased by 4% with no protein reduction. To assess other seed composition changes, seeds of two independent transgenic (VgD) soybean lines and controls were obtained from two field locations in multiple years. Many analyses addressed what seed component(s) was/were reduced to compensate for the oil increase, including carbohydrate analysis (structural and nonstructural), NDF (Neutral detergent fiber), main minerals and proximate analyses. In Princeton, oil increased by 4% for the high oil lines in 2016 and 2017, with no significant reduction in protein, this was slightly different in Spindletop with an increase in oil by 1.6–1.7 %, and an increase in protein by 2.6–2.4 % in comparison to the control, as an average for both high oil lines in 2016 and 2017. Soluble carbohydrate was reduced in VgD by 1.2 % in Princeton location for the high oil lines in 2016 and 2017, whereas only VgD1-2 was significantly reduced in Spindletop in comparison to Jack on 2016 with 2%, Oil in the VgD seeds was greater than in control seeds without a decrease in seed protein in both locations, suggesting that the trait was maintained across environments.

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Proximate composition of enhanced DGAT high oil, high protein soybeans

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

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